This study presents a novel antibacterial oxygen-producing silk fibroin methacryloyl hydrogel microneedle (MN) patch designed to enhance diabetic wound healing. The patch consists of tips encapsulated with calcium peroxide and catalase, and a base coated with antibacterial silver nanoparticles (AgNPs). The MN patch continuously releases oxygen, inhibits reactive oxygen species (ROS), and promotes cellular proliferation, migration, macrophage M2 polarization, and angiogenesis. The AgNPs at the base of the patch effectively combat microbial infections, further facilitating wound repair. The findings suggest that this multifunctional oxygen-producing MN patch is a promising strategy for diabetic wound healing in clinical settings. The study includes detailed characterization of the patch, in vitro and in vivo experiments, and assessments of biocompatibility, pro-angiogenic and antibacterial properties, and wound healing outcomes.This study presents a novel antibacterial oxygen-producing silk fibroin methacryloyl hydrogel microneedle (MN) patch designed to enhance diabetic wound healing. The patch consists of tips encapsulated with calcium peroxide and catalase, and a base coated with antibacterial silver nanoparticles (AgNPs). The MN patch continuously releases oxygen, inhibits reactive oxygen species (ROS), and promotes cellular proliferation, migration, macrophage M2 polarization, and angiogenesis. The AgNPs at the base of the patch effectively combat microbial infections, further facilitating wound repair. The findings suggest that this multifunctional oxygen-producing MN patch is a promising strategy for diabetic wound healing in clinical settings. The study includes detailed characterization of the patch, in vitro and in vivo experiments, and assessments of biocompatibility, pro-angiogenic and antibacterial properties, and wound healing outcomes.