A novel antibacterial oxygen-producing silk fibroin methacryloyl (SilMA) hydrogel microneedle (MN) patch was developed to enhance diabetic wound healing. The patch consists of microneedles encapsulated with calcium peroxide (CaO₂) and catalase, and a base coated with silver nanoparticles (AgNPs). The CaO₂ in the microneedle tips continuously releases oxygen, which helps alleviate hypoxia in diabetic wounds, promoting cellular growth, macrophage M2 polarization, and angiogenesis. The AgNPs on the base of the patch combat microbial infections, further aiding wound repair. The patch effectively reduces inflammation, improves oxygen supply, and accelerates healing. The hydrogel is biocompatible, stable, and capable of sustained oxygen release. The study demonstrates that this multifunctional MN patch is a promising strategy for treating diabetic wounds, offering good biocompatibility, multifunctionality, and high stability. The patch shows significant therapeutic effects in a mouse model of type 1 diabetes with infection, making it a potential treatment for diabetic wound healing and related biomedical applications.A novel antibacterial oxygen-producing silk fibroin methacryloyl (SilMA) hydrogel microneedle (MN) patch was developed to enhance diabetic wound healing. The patch consists of microneedles encapsulated with calcium peroxide (CaO₂) and catalase, and a base coated with silver nanoparticles (AgNPs). The CaO₂ in the microneedle tips continuously releases oxygen, which helps alleviate hypoxia in diabetic wounds, promoting cellular growth, macrophage M2 polarization, and angiogenesis. The AgNPs on the base of the patch combat microbial infections, further aiding wound repair. The patch effectively reduces inflammation, improves oxygen supply, and accelerates healing. The hydrogel is biocompatible, stable, and capable of sustained oxygen release. The study demonstrates that this multifunctional MN patch is a promising strategy for treating diabetic wounds, offering good biocompatibility, multifunctionality, and high stability. The patch shows significant therapeutic effects in a mouse model of type 1 diabetes with infection, making it a potential treatment for diabetic wound healing and related biomedical applications.