This study investigates the antifungal efficacy and cytotoxicity of Fe3O4@SiO2/Schiff-base/Cu(II) magnetic nanoparticles (MNPs) against drug-resistant Candida species. The MNPs were synthesized using a co-precipitation method and characterized by various techniques, including FT-IR, XRD, TEM, SEM, DLS, VSM, and TGA. The antifungal activity was evaluated using the broth microdilution method against six Candida species, with C. parapsilosis showing the lowest MIC (8 μg/mL) and C. albicans the highest (32 μg/mL). The antifungal mechanism is attributed to the disruption of fungal cell walls and membranes, along with increased reactive oxygen species (ROS) generation. The MTT assay demonstrated low toxicity and even enhanced cell proliferation at certain concentrations, suggesting potential applications in treating life-threatening fungal infections and healthcare-associated infections.This study investigates the antifungal efficacy and cytotoxicity of Fe3O4@SiO2/Schiff-base/Cu(II) magnetic nanoparticles (MNPs) against drug-resistant Candida species. The MNPs were synthesized using a co-precipitation method and characterized by various techniques, including FT-IR, XRD, TEM, SEM, DLS, VSM, and TGA. The antifungal activity was evaluated using the broth microdilution method against six Candida species, with C. parapsilosis showing the lowest MIC (8 μg/mL) and C. albicans the highest (32 μg/mL). The antifungal mechanism is attributed to the disruption of fungal cell walls and membranes, along with increased reactive oxygen species (ROS) generation. The MTT assay demonstrated low toxicity and even enhanced cell proliferation at certain concentrations, suggesting potential applications in treating life-threatening fungal infections and healthcare-associated infections.