A novel magnetic nanoparticle, Fe₃O₄@SiO₂/Schiff-base/Cu(II), was synthesized and evaluated for its antifungal activity against six pathogenic Candida species. The nanoparticles were characterized using various techniques, including FT-IR, XRD, TEM, SEM, DLS, EDX, VSM, and TGA, confirming successful synthesis. The antifungal activity was assessed using the broth microdilution method, revealing strong antifungal effects with MIC values ranging from 8 to 64 μg/mL. The lowest MIC was observed against C. parapsilosis (8 μg/mL), while the highest was against C. albicans (32 μg/mL). The antifungal mechanism is likely due to disruption of the fungal cell wall and membrane, along with increased reactive oxygen species (ROS) generation leading to cell death. Cytotoxicity tests on mouse L929 fibroblastic cells showed low toxicity and even enhanced cell proliferation at certain concentrations. The study highlights the potential of Fe₃O₄@SiO₂/Schiff-base/Cu(II) nanoparticles as a promising antifungal agent for treating fungal infections, including drug-resistant Candida species. The magnetic properties of the nanoparticles allow for targeted delivery, improving therapeutic efficacy and reducing toxicity. The results suggest that these nanoparticles could be a valuable alternative to traditional antifungal drugs, particularly in cases of resistance and persistent infections.A novel magnetic nanoparticle, Fe₃O₄@SiO₂/Schiff-base/Cu(II), was synthesized and evaluated for its antifungal activity against six pathogenic Candida species. The nanoparticles were characterized using various techniques, including FT-IR, XRD, TEM, SEM, DLS, EDX, VSM, and TGA, confirming successful synthesis. The antifungal activity was assessed using the broth microdilution method, revealing strong antifungal effects with MIC values ranging from 8 to 64 μg/mL. The lowest MIC was observed against C. parapsilosis (8 μg/mL), while the highest was against C. albicans (32 μg/mL). The antifungal mechanism is likely due to disruption of the fungal cell wall and membrane, along with increased reactive oxygen species (ROS) generation leading to cell death. Cytotoxicity tests on mouse L929 fibroblastic cells showed low toxicity and even enhanced cell proliferation at certain concentrations. The study highlights the potential of Fe₃O₄@SiO₂/Schiff-base/Cu(II) nanoparticles as a promising antifungal agent for treating fungal infections, including drug-resistant Candida species. The magnetic properties of the nanoparticles allow for targeted delivery, improving therapeutic efficacy and reducing toxicity. The results suggest that these nanoparticles could be a valuable alternative to traditional antifungal drugs, particularly in cases of resistance and persistent infections.