This chapter provides an overview of the importance of ants in biodiversity studies and monitoring programs. It highlights the ecological significance of ants, their high diversity, numerical dominance, and ease of collection, making them ideal for biodiversity research. The chapter discusses the benefits of including ants in biodiversity programs, such as their sensitivity to environmental changes and their role in ecosystem functioning. It also outlines the steps for incorporating ants into biodiversity studies, including background knowledge, sampling methods, and specimen identification. The chapter emphasizes the importance of collaboration with ant taxonomists and the value of data exchange between local researchers and ant specialists. Finally, it encourages the use of standardized protocols, such as the ALL Protocol, to ensure consistent and comparable data across different studies.This chapter provides an overview of the importance of ants in biodiversity studies and monitoring programs. It highlights the ecological significance of ants, their high diversity, numerical dominance, and ease of collection, making them ideal for biodiversity research. The chapter discusses the benefits of including ants in biodiversity programs, such as their sensitivity to environmental changes and their role in ecosystem functioning. It also outlines the steps for incorporating ants into biodiversity studies, including background knowledge, sampling methods, and specimen identification. The chapter emphasizes the importance of collaboration with ant taxonomists and the value of data exchange between local researchers and ant specialists. Finally, it encourages the use of standardized protocols, such as the ALL Protocol, to ensure consistent and comparable data across different studies.