Apoptosis-deficient mice show increased DNA damage and premature senescence, with reduced levels of apoptotic vesicles (apoVs). Intravenous infusion of mesenchymal stromal cell (MSC)-derived apoVs rescued DNA damage and premature senescence in these mice. Apoptosis generates apoVs containing DNA repair enzymes, which are transferred to recipient cells, aiding in DNA repair. Embryonic stem cell (ESC)-derived apoVs have superior DNA repair capacity and can rescue irradiation-induced DNA damage. This study reveals that apoVs play a critical role in DNA repair and tissue protection, offering a potential therapeutic strategy for radiation damage. The findings highlight the importance of apoptosis in DNA repair and the therapeutic potential of apoVs in treating irradiation-induced damage.Apoptosis-deficient mice show increased DNA damage and premature senescence, with reduced levels of apoptotic vesicles (apoVs). Intravenous infusion of mesenchymal stromal cell (MSC)-derived apoVs rescued DNA damage and premature senescence in these mice. Apoptosis generates apoVs containing DNA repair enzymes, which are transferred to recipient cells, aiding in DNA repair. Embryonic stem cell (ESC)-derived apoVs have superior DNA repair capacity and can rescue irradiation-induced DNA damage. This study reveals that apoVs play a critical role in DNA repair and tissue protection, offering a potential therapeutic strategy for radiation damage. The findings highlight the importance of apoptosis in DNA repair and the therapeutic potential of apoVs in treating irradiation-induced damage.