Artemis is a Java-based sequence visualization and annotation tool designed for analyzing compact genomes of bacteria, archaea, and lower eukaryotes. It can handle sequences of any size, from small genes to whole genomes, and supports EMBL, GenBank, and GFF formats. Artemis allows users to visualize and annotate sequences, plot sequence properties, and overlay external analysis results. It is available under the GNU General Public License and can be run on various platforms. The tool has been used extensively in the annotation of genomes, including *Campylobacter jejuni*, *Neisseria meningitidis*, and *Plasmodium falciparum*. Artemis is particularly useful for its dynamic plotting capabilities, interactive editing features, and web-based applet functionality.Artemis is a Java-based sequence visualization and annotation tool designed for analyzing compact genomes of bacteria, archaea, and lower eukaryotes. It can handle sequences of any size, from small genes to whole genomes, and supports EMBL, GenBank, and GFF formats. Artemis allows users to visualize and annotate sequences, plot sequence properties, and overlay external analysis results. It is available under the GNU General Public License and can be run on various platforms. The tool has been used extensively in the annotation of genomes, including *Campylobacter jejuni*, *Neisseria meningitidis*, and *Plasmodium falciparum*. Artemis is particularly useful for its dynamic plotting capabilities, interactive editing features, and web-based applet functionality.