This study evaluates the nutritional quality of Pleurotus ostreatus (oyster mushroom), highlighting its potential as a functional food. The mushroom was analyzed for proximate composition, vitamins, minerals, and amino acids. The proximate analysis revealed that Pleurotus ostreatus contains 43.42% carbohydrates, 23.63% crude fiber, 17.06% crude protein, 8.22% ash, 1.21% lipid, and 91.01% moisture in fresh samples, with 6.46% moisture in dry samples. The monosaccharide and disaccharide profile showed high levels of glucose, fructose, and sucrose, while the amino acid composition indicated a higher presence of non-essential amino acids compared to essential ones. The mushroom was also rich in potassium, iron, and magnesium, and contained significant amounts of vitamins A, C, E, and B vitamins, with vitamin B2 being the highest. The study concludes that Pleurotus ostreatus is a nutrient-rich functional food that can help address malnutrition and micronutrient deficiencies, especially in developing countries. The findings suggest that this mushroom can be a valuable addition to the diet for its health benefits.This study evaluates the nutritional quality of Pleurotus ostreatus (oyster mushroom), highlighting its potential as a functional food. The mushroom was analyzed for proximate composition, vitamins, minerals, and amino acids. The proximate analysis revealed that Pleurotus ostreatus contains 43.42% carbohydrates, 23.63% crude fiber, 17.06% crude protein, 8.22% ash, 1.21% lipid, and 91.01% moisture in fresh samples, with 6.46% moisture in dry samples. The monosaccharide and disaccharide profile showed high levels of glucose, fructose, and sucrose, while the amino acid composition indicated a higher presence of non-essential amino acids compared to essential ones. The mushroom was also rich in potassium, iron, and magnesium, and contained significant amounts of vitamins A, C, E, and B vitamins, with vitamin B2 being the highest. The study concludes that Pleurotus ostreatus is a nutrient-rich functional food that can help address malnutrition and micronutrient deficiencies, especially in developing countries. The findings suggest that this mushroom can be a valuable addition to the diet for its health benefits.