The study investigates the use of quorum sensing inhibitors (QSIs) to attenuate the virulence of *Pseudomonas aeruginosa*, an opportunistic human pathogen. The researchers identified a synthetic halogenated furanone compound, C-30, which effectively inhibits quorum sensing and reduces the production of virulence factors such as protease, pyoverdin, and chitinase. Microarray analysis revealed that C-30 targets a subset of genes involved in quorum sensing, particularly those encoding major virulence factors. In vitro and in vivo experiments demonstrated that C-30 enhances the susceptibility of *P. aeruginosa* biofilms to antibiotics and promotes the clearance of infecting bacteria in a mouse model of pulmonary infection. The findings suggest that QSIs represent a novel approach to control bacterial infections by specifically blocking cell-to-cell communication without affecting bacterial growth.The study investigates the use of quorum sensing inhibitors (QSIs) to attenuate the virulence of *Pseudomonas aeruginosa*, an opportunistic human pathogen. The researchers identified a synthetic halogenated furanone compound, C-30, which effectively inhibits quorum sensing and reduces the production of virulence factors such as protease, pyoverdin, and chitinase. Microarray analysis revealed that C-30 targets a subset of genes involved in quorum sensing, particularly those encoding major virulence factors. In vitro and in vivo experiments demonstrated that C-30 enhances the susceptibility of *P. aeruginosa* biofilms to antibiotics and promotes the clearance of infecting bacteria in a mouse model of pulmonary infection. The findings suggest that QSIs represent a novel approach to control bacterial infections by specifically blocking cell-to-cell communication without affecting bacterial growth.