The Dual Organellar GenoMe Annotator (DOGMA) is a web-based tool designed to automate the annotation of organellar genomes, specifically plant chloroplasts and animal mitochondria. It uses BLAST searches against custom databases to identify and annotate genes, including protein-coding genes, ribosomal RNAs (rRNAs), and transfer RNAs (tRNAs). DOGMA provides a graphical user interface for viewing and editing annotations, which are stored on a password-protected server for repeated use. The tool allows users to extract finished annotations for submission to GenBank. The database for animal mitochondrial genomes includes 37 genes, while the chloroplast genome database covers 110-130 genes. DOGMA also addresses the challenges of annotating tRNAs in animal mitochondria by using hidden Markov models and inferring secondary structures. Future developments aim to expand the database to include more taxa and add features such as RNA editing and an ORF finder.The Dual Organellar GenoMe Annotator (DOGMA) is a web-based tool designed to automate the annotation of organellar genomes, specifically plant chloroplasts and animal mitochondria. It uses BLAST searches against custom databases to identify and annotate genes, including protein-coding genes, ribosomal RNAs (rRNAs), and transfer RNAs (tRNAs). DOGMA provides a graphical user interface for viewing and editing annotations, which are stored on a password-protected server for repeated use. The tool allows users to extract finished annotations for submission to GenBank. The database for animal mitochondrial genomes includes 37 genes, while the chloroplast genome database covers 110-130 genes. DOGMA also addresses the challenges of annotating tRNAs in animal mitochondria by using hidden Markov models and inferring secondary structures. Future developments aim to expand the database to include more taxa and add features such as RNA editing and an ORF finder.