BLAST+ is a new version of the BLAST software, designed to improve performance and usability. The software includes new command-line applications and features such as query splitting, partial retrieval of subject sequences, and database masking. These improvements allow for faster searches, especially for long queries and large databases. The new BLAST+ applications are more efficient and user-friendly compared to the previous versions. The software is available for use on various platforms, including Linux, UNIX, and Windows. The BLAST+ applications can be used for a variety of sequence comparison tasks, including nucleotide-nucleotide, nucleotide-protein, and protein-protein searches. The software also includes a new modular library that allows for the retrieval of subject sequences from arbitrary data sources. The new BLAST+ applications are supported by the NCBI C++ toolkit and the NCBI C toolkit. The software is available under an open access license, allowing free use and distribution. The new BLAST+ applications have been tested and shown to improve performance in various scenarios, including searches against large databases and long queries. The software is supported by the National Institutes of Health and is available for public use.BLAST+ is a new version of the BLAST software, designed to improve performance and usability. The software includes new command-line applications and features such as query splitting, partial retrieval of subject sequences, and database masking. These improvements allow for faster searches, especially for long queries and large databases. The new BLAST+ applications are more efficient and user-friendly compared to the previous versions. The software is available for use on various platforms, including Linux, UNIX, and Windows. The BLAST+ applications can be used for a variety of sequence comparison tasks, including nucleotide-nucleotide, nucleotide-protein, and protein-protein searches. The software also includes a new modular library that allows for the retrieval of subject sequences from arbitrary data sources. The new BLAST+ applications are supported by the NCBI C++ toolkit and the NCBI C toolkit. The software is available under an open access license, allowing free use and distribution. The new BLAST+ applications have been tested and shown to improve performance in various scenarios, including searches against large databases and long queries. The software is supported by the National Institutes of Health and is available for public use.