Chronic wounds are injuries that fail to heal properly and are often infected, posing significant health and economic challenges. Bacterial biofilms, which are complex structures formed by bacteria, hinder treatment effectiveness and prolong healing. This review discusses the wound healing process, the pathogenesis of chronic wounds, and therapeutic strategies to combat biofilm-related infections. It highlights the role of the skin microbiota in wound healing and presents various approaches to disrupt biofilm formation or destroy existing biofilms. These strategies include debridement, antimicrobial agents, probiotics, bacteriophages, and QS inhibitors. The review also emphasizes the importance of restoring the skin microbiota to promote healing and reduce chronic inflammation. New therapeutic approaches, such as the use of antimicrobial peptides, nanoparticles, and immunotherapies, are explored as potential solutions to improve wound healing and reduce the impact of chronic wounds on public health. The study underscores the need for further research to develop effective treatments for chronic wounds and to enhance the management of biofilm-related infections.Chronic wounds are injuries that fail to heal properly and are often infected, posing significant health and economic challenges. Bacterial biofilms, which are complex structures formed by bacteria, hinder treatment effectiveness and prolong healing. This review discusses the wound healing process, the pathogenesis of chronic wounds, and therapeutic strategies to combat biofilm-related infections. It highlights the role of the skin microbiota in wound healing and presents various approaches to disrupt biofilm formation or destroy existing biofilms. These strategies include debridement, antimicrobial agents, probiotics, bacteriophages, and QS inhibitors. The review also emphasizes the importance of restoring the skin microbiota to promote healing and reduce chronic inflammation. New therapeutic approaches, such as the use of antimicrobial peptides, nanoparticles, and immunotherapies, are explored as potential solutions to improve wound healing and reduce the impact of chronic wounds on public health. The study underscores the need for further research to develop effective treatments for chronic wounds and to enhance the management of biofilm-related infections.