Chronic wounds, which fail to progress through the normal healing process, are a significant health issue due to their frequent association with infections and the challenges they pose in treatment. The ability of pathogens to form multicellular structures called biofilms hinders the efficacy of treatments. This review aims to enhance understanding of the pathophysiology and treatment of infected chronic wounds. It provides a comprehensive overview of the wound healing process, the pathogenesis of chronic wounds, and the role of bacterial biofilms and skin microbiota. The review also discusses current and novel therapeutic strategies to counteract biofilm formation and promote wound healing, including mechanical debridement, use of probiotics, anti-quorum sensing (QS) molecules, bacteriophages, nanoparticles, and immunotherapies. The combination of these strategies has the potential to improve patient outcomes and reduce serious healthcare complications. However, further large-scale studies are needed to validate these approaches.Chronic wounds, which fail to progress through the normal healing process, are a significant health issue due to their frequent association with infections and the challenges they pose in treatment. The ability of pathogens to form multicellular structures called biofilms hinders the efficacy of treatments. This review aims to enhance understanding of the pathophysiology and treatment of infected chronic wounds. It provides a comprehensive overview of the wound healing process, the pathogenesis of chronic wounds, and the role of bacterial biofilms and skin microbiota. The review also discusses current and novel therapeutic strategies to counteract biofilm formation and promote wound healing, including mechanical debridement, use of probiotics, anti-quorum sensing (QS) molecules, bacteriophages, nanoparticles, and immunotherapies. The combination of these strategies has the potential to improve patient outcomes and reduce serious healthcare complications. However, further large-scale studies are needed to validate these approaches.