Bacterial efflux pump inhibitors reduce antibiotic resistance by blocking the efflux of antibiotics, thereby increasing their intracellular concentration and restoring their effectiveness. This review discusses the mechanisms of bacterial resistance, the role of efflux pumps in antibiotic resistance, and the potential of efflux pump inhibitors in combating drug resistance. Bacterial resistance is a growing global problem, with antibiotic resistance leading to increased mortality and economic losses. Efflux pumps are a major factor in antibiotic resistance, and their inhibition can help reduce resistance. Efflux pump inhibitors, which can be natural or synthetic, have shown promise in reversing drug resistance. They work by increasing the intracellular accumulation of antibiotics, making bacteria more susceptible to antimicrobial drugs. Efflux pump inhibitors have been tested against various pathogens, including Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and Campylobacter jejuni. These inhibitors have shown synergistic effects with antibiotics, reducing the minimum inhibitory concentration (MIC) of antibiotics and enhancing their efficacy. Efflux pump inhibitors also reduce biofilm formation, bacterial virulence, and the formation of bacterial persister cells. They limit the acquisition of bacterial resistance by inhibiting the transfer of resistance genes. Overall, efflux pump inhibitors have significant potential in addressing and reducing bacterial resistance.Bacterial efflux pump inhibitors reduce antibiotic resistance by blocking the efflux of antibiotics, thereby increasing their intracellular concentration and restoring their effectiveness. This review discusses the mechanisms of bacterial resistance, the role of efflux pumps in antibiotic resistance, and the potential of efflux pump inhibitors in combating drug resistance. Bacterial resistance is a growing global problem, with antibiotic resistance leading to increased mortality and economic losses. Efflux pumps are a major factor in antibiotic resistance, and their inhibition can help reduce resistance. Efflux pump inhibitors, which can be natural or synthetic, have shown promise in reversing drug resistance. They work by increasing the intracellular accumulation of antibiotics, making bacteria more susceptible to antimicrobial drugs. Efflux pump inhibitors have been tested against various pathogens, including Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and Campylobacter jejuni. These inhibitors have shown synergistic effects with antibiotics, reducing the minimum inhibitory concentration (MIC) of antibiotics and enhancing their efficacy. Efflux pump inhibitors also reduce biofilm formation, bacterial virulence, and the formation of bacterial persister cells. They limit the acquisition of bacterial resistance by inhibiting the transfer of resistance genes. Overall, efflux pump inhibitors have significant potential in addressing and reducing bacterial resistance.