The article provides an updated classification and overview of bacterial lipolytic enzymes, including carboxylesterases and true lipases. The authors have compiled and classified information from protein and nucleotide databases to propose a comprehensive classification based on amino acid sequences and biological properties. This classification identifies eight families, with the largest family further divided into six subfamilies. The classification helps predict structural features, secretion mechanisms, and potential relationships with other enzyme families. The study also highlights the biotechnological potential of these enzymes, particularly in industrial applications. The article discusses the structural and functional characteristics of various bacterial lipases and esterases, emphasizing their diverse properties and relatedness to other protein families. The authors aim to provide a basis for further research and characterization of novel bacterial lipolytic enzymes.The article provides an updated classification and overview of bacterial lipolytic enzymes, including carboxylesterases and true lipases. The authors have compiled and classified information from protein and nucleotide databases to propose a comprehensive classification based on amino acid sequences and biological properties. This classification identifies eight families, with the largest family further divided into six subfamilies. The classification helps predict structural features, secretion mechanisms, and potential relationships with other enzyme families. The study also highlights the biotechnological potential of these enzymes, particularly in industrial applications. The article discusses the structural and functional characteristics of various bacterial lipases and esterases, emphasizing their diverse properties and relatedness to other protein families. The authors aim to provide a basis for further research and characterization of novel bacterial lipolytic enzymes.