A systematic review of barriers to cervical cancer screening in Africa identified multiple factors preventing women from accessing screening services. These barriers include poor access to screening facilities, lack of awareness and knowledge about cervical cancer and screening, socio-cultural influences, and limited resources such as trained personnel, equipment, and supplies. Service providers also reported challenges such as lack of training, insufficient funding, and gender and age-related biases affecting screening provision. At the individual level, women often lack awareness of cervical cancer, financial constraints, and negative attitudes towards screening. Interpersonal barriers include lack of spousal support and misconceptions about cervical cancer. Community-level barriers involve cultural and religious beliefs, gender and age of service providers, and social stigma. Structural barriers include inadequate policies, weak implementation, and limited funding for screening programs. The review highlights the need for a holistic approach addressing barriers at individual, interpersonal, community, health system, and structural levels. Strategies such as improving access to screening services, increasing awareness through community mobilization, and ensuring trained personnel and adequate resources are recommended. The study emphasizes the importance of addressing socio-ecological factors to improve screening uptake and reduce cervical cancer morbidity and mortality in Africa. The findings suggest that current efforts are fragmented and insufficient, and a coordinated approach is needed to effectively address the multifaceted barriers to cervical cancer screening.A systematic review of barriers to cervical cancer screening in Africa identified multiple factors preventing women from accessing screening services. These barriers include poor access to screening facilities, lack of awareness and knowledge about cervical cancer and screening, socio-cultural influences, and limited resources such as trained personnel, equipment, and supplies. Service providers also reported challenges such as lack of training, insufficient funding, and gender and age-related biases affecting screening provision. At the individual level, women often lack awareness of cervical cancer, financial constraints, and negative attitudes towards screening. Interpersonal barriers include lack of spousal support and misconceptions about cervical cancer. Community-level barriers involve cultural and religious beliefs, gender and age of service providers, and social stigma. Structural barriers include inadequate policies, weak implementation, and limited funding for screening programs. The review highlights the need for a holistic approach addressing barriers at individual, interpersonal, community, health system, and structural levels. Strategies such as improving access to screening services, increasing awareness through community mobilization, and ensuring trained personnel and adequate resources are recommended. The study emphasizes the importance of addressing socio-ecological factors to improve screening uptake and reduce cervical cancer morbidity and mortality in Africa. The findings suggest that current efforts are fragmented and insufficient, and a coordinated approach is needed to effectively address the multifaceted barriers to cervical cancer screening.