This systematic review examines the barriers to cervical cancer screening in Africa, a region with one of the highest burdens of cervical cancer. The review aims to identify factors that prevent women from utilizing cervical cancer screening services, which are crucial for reducing incidence and mortality rates. A mixed-methods approach was used, including qualitative, quantitative, and mixed-method studies published up to May 2019. The studies were selected from databases such as PubMed, EMBASE, CINAHL, and Scopus, and appraised for quality using standard criteria. The primary barriers identified include poor access to screening services, lack of awareness and knowledge about cervical cancer and screening, and socio-cultural influences. Service providers also reported challenges such as lack of skills, insufficient equipment and supplies, and staff shortages. The review found that these barriers are multifaceted and require a holistic approach to address them at multiple levels: individual, interpersonal, community, health system, and structural. The review suggests that increasing access to screening services, improving awareness and knowledge, addressing socio-cultural barriers, and ensuring adequate resources and training for health providers are essential for improving cervical cancer screening rates in Africa. Political will and stakeholder involvement are crucial for developing and implementing effective strategies to ensure the acceptability, availability, accessibility, and affordability of screening services.This systematic review examines the barriers to cervical cancer screening in Africa, a region with one of the highest burdens of cervical cancer. The review aims to identify factors that prevent women from utilizing cervical cancer screening services, which are crucial for reducing incidence and mortality rates. A mixed-methods approach was used, including qualitative, quantitative, and mixed-method studies published up to May 2019. The studies were selected from databases such as PubMed, EMBASE, CINAHL, and Scopus, and appraised for quality using standard criteria. The primary barriers identified include poor access to screening services, lack of awareness and knowledge about cervical cancer and screening, and socio-cultural influences. Service providers also reported challenges such as lack of skills, insufficient equipment and supplies, and staff shortages. The review found that these barriers are multifaceted and require a holistic approach to address them at multiple levels: individual, interpersonal, community, health system, and structural. The review suggests that increasing access to screening services, improving awareness and knowledge, addressing socio-cultural barriers, and ensuring adequate resources and training for health providers are essential for improving cervical cancer screening rates in Africa. Political will and stakeholder involvement are crucial for developing and implementing effective strategies to ensure the acceptability, availability, accessibility, and affordability of screening services.