The book "Basic Plant Pathology Methods" by Onkar D. Dhingra and James B. Sinclair is a comprehensive guide to the techniques and methods used in plant pathology. The second edition, published in 2019, aims to serve as a practical reference for both classroom and laboratory use, particularly for those in developing countries where access to advanced library resources may be limited. The authors emphasize the importance of basic techniques in solving plant disease problems and provide detailed descriptions of various methods, including sterilization of apparatus and culture media, culture of pathogens, long-term storage of pathogens, detection and estimation of inoculum, fungicide evaluation, biological control, and microscopy techniques. The book also includes appendices with formulas for culture media, solutions for maintaining humidity, drying agents, pH indicator dyes, and other useful information. The authors, both experienced plant pathologists, have contributed their expertise to ensure the accuracy and实用性 of the content.The book "Basic Plant Pathology Methods" by Onkar D. Dhingra and James B. Sinclair is a comprehensive guide to the techniques and methods used in plant pathology. The second edition, published in 2019, aims to serve as a practical reference for both classroom and laboratory use, particularly for those in developing countries where access to advanced library resources may be limited. The authors emphasize the importance of basic techniques in solving plant disease problems and provide detailed descriptions of various methods, including sterilization of apparatus and culture media, culture of pathogens, long-term storage of pathogens, detection and estimation of inoculum, fungicide evaluation, biological control, and microscopy techniques. The book also includes appendices with formulas for culture media, solutions for maintaining humidity, drying agents, pH indicator dyes, and other useful information. The authors, both experienced plant pathologists, have contributed their expertise to ensure the accuracy and实用性 of the content.