This systematic review examines the integration of bicycle infrastructure design principles into urban bikeability indices (BIs). The review analyzed 15 studies published between 2010 and 2023, focusing on how BIs incorporate bicycle infrastructure design principles such as safety, comfort, attractiveness, directness, and coherence. The findings reveal that while bicycle infrastructure is the most commonly considered factor in BIs, only three studies considered all five principles. Safety and comfort are the most frequently included, while coherence is the least considered. The review highlights the importance of considering all five principles to create a bicycle-friendly environment and promote sustainable transportation. The studies used various methods to develop BIs, including scoring and weighting systems, expert surveys, and objective data analysis. The unit of analysis varied, ranging from street segments to city-wide assessments. The review also identified key indicators such as bicycle infrastructure, greenery, slope, traffic volume, and street lighting as critical for assessing bikeability. The results emphasize the need for a comprehensive approach to bikeability assessment that incorporates all five design principles to ensure a safe, comfortable, and attractive cycling environment. The review concludes that future research should focus on developing BIs that incorporate all five principles for a more accurate and holistic assessment of bikeability.This systematic review examines the integration of bicycle infrastructure design principles into urban bikeability indices (BIs). The review analyzed 15 studies published between 2010 and 2023, focusing on how BIs incorporate bicycle infrastructure design principles such as safety, comfort, attractiveness, directness, and coherence. The findings reveal that while bicycle infrastructure is the most commonly considered factor in BIs, only three studies considered all five principles. Safety and comfort are the most frequently included, while coherence is the least considered. The review highlights the importance of considering all five principles to create a bicycle-friendly environment and promote sustainable transportation. The studies used various methods to develop BIs, including scoring and weighting systems, expert surveys, and objective data analysis. The unit of analysis varied, ranging from street segments to city-wide assessments. The review also identified key indicators such as bicycle infrastructure, greenery, slope, traffic volume, and street lighting as critical for assessing bikeability. The results emphasize the need for a comprehensive approach to bikeability assessment that incorporates all five design principles to ensure a safe, comfortable, and attractive cycling environment. The review concludes that future research should focus on developing BIs that incorporate all five principles for a more accurate and holistic assessment of bikeability.