This paper presents a systematic literature review of urban bikeability indices (BIs) to understand their development and the consideration of bicycle infrastructure design principles. The review aims to identify essential indicators and methods used in BIs, as well as the unit of analysis and data sources. The study found that 15 out of 1649 research articles met the inclusion criteria, with studies conducted in various geographical locations. The most common method for developing BIs was a scoring and weighting system, using methods such as equal weight, survey-based, literature review-based, expert surveys, analytic hierarchy process, and weighted linear combination models. Bicycle infrastructure, including bike lanes, routes, and paths, was the most commonly considered criterion in 14 studies. Safety and comfort were the most frequently considered principles, while coherence was the least considered. The review highlights the need for a comprehensive approach that considers all five bicycle infrastructure design principles (safety, comfort, attractiveness, directness, and coherence) to create a holistic and effective BI. Future research should develop a BI that incorporates all indicators to provide a more accurate assessment of bikeability and guide policymakers and urban planners in creating safer and more attractive cycling environments.This paper presents a systematic literature review of urban bikeability indices (BIs) to understand their development and the consideration of bicycle infrastructure design principles. The review aims to identify essential indicators and methods used in BIs, as well as the unit of analysis and data sources. The study found that 15 out of 1649 research articles met the inclusion criteria, with studies conducted in various geographical locations. The most common method for developing BIs was a scoring and weighting system, using methods such as equal weight, survey-based, literature review-based, expert surveys, analytic hierarchy process, and weighted linear combination models. Bicycle infrastructure, including bike lanes, routes, and paths, was the most commonly considered criterion in 14 studies. Safety and comfort were the most frequently considered principles, while coherence was the least considered. The review highlights the need for a comprehensive approach that considers all five bicycle infrastructure design principles (safety, comfort, attractiveness, directness, and coherence) to create a holistic and effective BI. Future research should develop a BI that incorporates all indicators to provide a more accurate assessment of bikeability and guide policymakers and urban planners in creating safer and more attractive cycling environments.