The article by Michael Bevan describes the construction and use of a binary Agrobacterium vector for efficient plant transformation. The vector utilizes the trans-acting functions of the vir region on a Ti plasmid to transfer a modified T-DNA region into plant cells. The modified T-DNA contains only border repeats and a chimeric nopaline synthase-neomycin phosphotransferase gene, which confers high levels of kanamycin resistance. The vector is designed to be efficient and user-friendly, with multiple cloning sites and unique restriction sites for positive selection of inserted DNA. The vector's efficiency is demonstrated through successful transformation experiments in Nicotiana plumbaginifolia, where it allows for the regeneration of fertile plants. The study also highlights the stability of the vector components and the ease of integration into plant DNA, making it a valuable tool for plant genetic engineering.The article by Michael Bevan describes the construction and use of a binary Agrobacterium vector for efficient plant transformation. The vector utilizes the trans-acting functions of the vir region on a Ti plasmid to transfer a modified T-DNA region into plant cells. The modified T-DNA contains only border repeats and a chimeric nopaline synthase-neomycin phosphotransferase gene, which confers high levels of kanamycin resistance. The vector is designed to be efficient and user-friendly, with multiple cloning sites and unique restriction sites for positive selection of inserted DNA. The vector's efficiency is demonstrated through successful transformation experiments in Nicotiana plumbaginifolia, where it allows for the regeneration of fertile plants. The study also highlights the stability of the vector components and the ease of integration into plant DNA, making it a valuable tool for plant genetic engineering.