This article presents a groundbreaking perspective on carotenoids, focusing on their innovative applications and transformative potential in human health and medicine. Research explores the bioactivity and bioavailability of carotenoids, revealing therapeutic uses and technological advances that could revolutionize medical treatments. The study highlights pioneering therapeutic applications in treating chronic diseases such as cancer, cardiovascular disease, and age-related macular degeneration, offering novel protective mechanisms and innovative therapeutic benefits. It also showcases cutting-edge technological innovations in carotenoid extraction and bioavailability, including the development of supramolecular carriers and advanced nanotechnology, which significantly improve the absorption and efficacy of these compounds. These advancements ensure consistent quality and tailor carotenoid therapies to individual patient needs, paving the way for personalized medicine. By integrating the latest scientific discoveries and innovative techniques, this research provides a prospective perspective on the clinical applications of carotenoids, establishing a new benchmark for future studies. The findings emphasize the importance of optimizing carotenoid extraction, administration, bioactivity, and bioavailability methods to develop more effective, targeted, and personalized treatments, offering visionary insight into their potential in modern medical practices. Carotenoids are a group of fat-soluble organic pigments synthesized by various organisms, with over 1117 structures identified from 683 different sources. They exhibit biological activities such as antioxidant properties, anti-inflammatory effects, and provitamin A attributes, making them valuable for medical and therapeutic applications. Carotenoids are classified into carotenes and xanthophylls, with carotenes including β-carotene, α-carotene, γ-carotene, δ-carotene, lycopene, neurosporene, and torulene, and xanthophylls including astaxanthin, zeaxanthin, lutein, torularhdin, canthaxanthin, and violaxanthin. These compounds play crucial roles in maintaining human organ functions, including eye, cardiovascular, and skin health, immune function, cognitive function, and bone health. Their mechanisms involve neutralizing free radicals, modulating gene expression, and regulating inflammatory pathways. Natural sources of carotenoids include brown algae, microalgae, and by-products of various foods. These sources offer promising prospects for the extraction of valuable carotenoids applicable in traditional industries and the pharmaceutical sector. The objective of this research is to document advances in carotenoid extraction techniques, highlighting their impact in medicine and the pharmaceutical industry. The article features innovative methodologies and technological advances in carotenoid extraction, focusing on improving their bioactivity and bioavailability for human health applications. The transformative potential of these techniques is highlighted by detailing how they improve efficiency, sustainability, and quality of carotenoid extraction. The article discusses various carotenoid extraction methods, including supercritical fluid extraction, pressurized liquid extraction, ionic liquid extraction, ultrasound-assisted extraction, microwave-assisted extraction, enzyme-assisted extraction, pulsed electricThis article presents a groundbreaking perspective on carotenoids, focusing on their innovative applications and transformative potential in human health and medicine. Research explores the bioactivity and bioavailability of carotenoids, revealing therapeutic uses and technological advances that could revolutionize medical treatments. The study highlights pioneering therapeutic applications in treating chronic diseases such as cancer, cardiovascular disease, and age-related macular degeneration, offering novel protective mechanisms and innovative therapeutic benefits. It also showcases cutting-edge technological innovations in carotenoid extraction and bioavailability, including the development of supramolecular carriers and advanced nanotechnology, which significantly improve the absorption and efficacy of these compounds. These advancements ensure consistent quality and tailor carotenoid therapies to individual patient needs, paving the way for personalized medicine. By integrating the latest scientific discoveries and innovative techniques, this research provides a prospective perspective on the clinical applications of carotenoids, establishing a new benchmark for future studies. The findings emphasize the importance of optimizing carotenoid extraction, administration, bioactivity, and bioavailability methods to develop more effective, targeted, and personalized treatments, offering visionary insight into their potential in modern medical practices. Carotenoids are a group of fat-soluble organic pigments synthesized by various organisms, with over 1117 structures identified from 683 different sources. They exhibit biological activities such as antioxidant properties, anti-inflammatory effects, and provitamin A attributes, making them valuable for medical and therapeutic applications. Carotenoids are classified into carotenes and xanthophylls, with carotenes including β-carotene, α-carotene, γ-carotene, δ-carotene, lycopene, neurosporene, and torulene, and xanthophylls including astaxanthin, zeaxanthin, lutein, torularhdin, canthaxanthin, and violaxanthin. These compounds play crucial roles in maintaining human organ functions, including eye, cardiovascular, and skin health, immune function, cognitive function, and bone health. Their mechanisms involve neutralizing free radicals, modulating gene expression, and regulating inflammatory pathways. Natural sources of carotenoids include brown algae, microalgae, and by-products of various foods. These sources offer promising prospects for the extraction of valuable carotenoids applicable in traditional industries and the pharmaceutical sector. The objective of this research is to document advances in carotenoid extraction techniques, highlighting their impact in medicine and the pharmaceutical industry. The article features innovative methodologies and technological advances in carotenoid extraction, focusing on improving their bioactivity and bioavailability for human health applications. The transformative potential of these techniques is highlighted by detailing how they improve efficiency, sustainability, and quality of carotenoid extraction. The article discusses various carotenoid extraction methods, including supercritical fluid extraction, pressurized liquid extraction, ionic liquid extraction, ultrasound-assisted extraction, microwave-assisted extraction, enzyme-assisted extraction, pulsed electric