This article provides a groundbreaking perspective on the innovative applications and transformative potential of carotenoids in human health and medicine. It delves into the bioactivity and bioavailability of carotenoids, highlighting their therapeutic uses and technological advancements that could revolutionize medical treatments. The study explores pioneering therapeutic applications of carotenoids in treating chronic diseases such as cancer, cardiovascular disease, and age-related macular degeneration, offering novel protective mechanisms and therapeutic benefits. Additionally, it showcases cutting-edge technological innovations in carotenoid extraction and bioavailability, including the development of supramolecular carriers and advanced nanotechnology, which significantly improve the absorption and efficacy of these compounds. These advancements ensure consistent quality and tailor carotenoid therapies to individual health needs, paving the way for personalized medicine. By integrating the latest scientific discoveries and innovative techniques, this research sets a new benchmark for future studies in this field, emphasizing the importance of optimizing carotenoid extraction, administration, bioactivity, and bioavailability methods to develop more effective, targeted, and personalized treatments.This article provides a groundbreaking perspective on the innovative applications and transformative potential of carotenoids in human health and medicine. It delves into the bioactivity and bioavailability of carotenoids, highlighting their therapeutic uses and technological advancements that could revolutionize medical treatments. The study explores pioneering therapeutic applications of carotenoids in treating chronic diseases such as cancer, cardiovascular disease, and age-related macular degeneration, offering novel protective mechanisms and therapeutic benefits. Additionally, it showcases cutting-edge technological innovations in carotenoid extraction and bioavailability, including the development of supramolecular carriers and advanced nanotechnology, which significantly improve the absorption and efficacy of these compounds. These advancements ensure consistent quality and tailor carotenoid therapies to individual health needs, paving the way for personalized medicine. By integrating the latest scientific discoveries and innovative techniques, this research sets a new benchmark for future studies in this field, emphasizing the importance of optimizing carotenoid extraction, administration, bioactivity, and bioavailability methods to develop more effective, targeted, and personalized treatments.