The paper investigates the biological properties of Poly(Lactide)–Copper composite materials, which were created by sputter deposition of copper onto Poly(lactide) melt-blown non-woven fabrics. The composite materials were tested for their antimicrobial and antifungal activities against various bacteria and fungi, as well as their biochemical-hematological properties, including the evaluation of activated partial thromboplastin time (aPTT), prothrombin time (PT), thrombin time (TT), and electron microscopy fibrin network imaging. The unmodified Poly(Lactide) fabric accelerated human blood plasma clotting in the intrinsic pathway, while copper plating abolished this effect. The study found that the unmodified PLA could be used for wound dressing materials, accelerating coagulation in hemorrhages, and its modifications with various metals might be applied as new materials where blood coagulation can be controlled, providing additional anti-pathogen effects. The substantial antimicrobial and antifungal activities of the PLA–Cu composites suggest potential applications as antibacterial/antifungal materials.The paper investigates the biological properties of Poly(Lactide)–Copper composite materials, which were created by sputter deposition of copper onto Poly(lactide) melt-blown non-woven fabrics. The composite materials were tested for their antimicrobial and antifungal activities against various bacteria and fungi, as well as their biochemical-hematological properties, including the evaluation of activated partial thromboplastin time (aPTT), prothrombin time (PT), thrombin time (TT), and electron microscopy fibrin network imaging. The unmodified Poly(Lactide) fabric accelerated human blood plasma clotting in the intrinsic pathway, while copper plating abolished this effect. The study found that the unmodified PLA could be used for wound dressing materials, accelerating coagulation in hemorrhages, and its modifications with various metals might be applied as new materials where blood coagulation can be controlled, providing additional anti-pathogen effects. The substantial antimicrobial and antifungal activities of the PLA–Cu composites suggest potential applications as antibacterial/antifungal materials.