This study investigates the biological properties of a Poly(Lactide)–Copper (PLA–Cu) composite material, created by depositing copper onto melt-blown nonwoven PLA fabrics using magnetron sputtering. The composite was tested for antimicrobial and antifungal activity against Gram-positive (Staphylococcus aureus), Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria, and fungal species (Chaetomium globosum, Candida albicans). Additionally, biochemical-hematological tests were conducted, including assessments of Activated Partial Thromboplastin Time (aPTT), Prothrombin Time (PT), and Thrombin Time (TT), as well as electron microscopy imaging of fibrin networks. The results showed that the PLA–Cu composite exhibited significant antimicrobial and antifungal properties, suggesting potential applications as an antibacterial/antifungal material. Unmodified PLA fabric was found to accelerate blood clotting in the intrinsic pathway, while copper plating eliminated this effect. The modified PLA–Cu composites showed varying effects on blood clotting, with PLA–Cu (10) causing a statistically significant prolongation of aPTT, while PLA–Cu (20) showed only minor differences compared to plasma alone. The composites did not significantly affect PT or TT, indicating that they do not interfere with the extrinsic pathway of blood coagulation. The results suggest that PLA–Cu composites could be used as wound dressings, accelerating coagulation in cases of hemorrhage, and their modifications with various metals could be used to control blood coagulation and provide additional anti-pathogen effects. The study highlights the potential of PLA–Cu composites in medical applications, particularly in wound healing and blood coagulation control.This study investigates the biological properties of a Poly(Lactide)–Copper (PLA–Cu) composite material, created by depositing copper onto melt-blown nonwoven PLA fabrics using magnetron sputtering. The composite was tested for antimicrobial and antifungal activity against Gram-positive (Staphylococcus aureus), Gram-negative (Escherichia coli, Pseudomonas aeruginosa) bacteria, and fungal species (Chaetomium globosum, Candida albicans). Additionally, biochemical-hematological tests were conducted, including assessments of Activated Partial Thromboplastin Time (aPTT), Prothrombin Time (PT), and Thrombin Time (TT), as well as electron microscopy imaging of fibrin networks. The results showed that the PLA–Cu composite exhibited significant antimicrobial and antifungal properties, suggesting potential applications as an antibacterial/antifungal material. Unmodified PLA fabric was found to accelerate blood clotting in the intrinsic pathway, while copper plating eliminated this effect. The modified PLA–Cu composites showed varying effects on blood clotting, with PLA–Cu (10) causing a statistically significant prolongation of aPTT, while PLA–Cu (20) showed only minor differences compared to plasma alone. The composites did not significantly affect PT or TT, indicating that they do not interfere with the extrinsic pathway of blood coagulation. The results suggest that PLA–Cu composites could be used as wound dressings, accelerating coagulation in cases of hemorrhage, and their modifications with various metals could be used to control blood coagulation and provide additional anti-pathogen effects. The study highlights the potential of PLA–Cu composites in medical applications, particularly in wound healing and blood coagulation control.