This review summarizes the biological activities of polyphenols from grapes, focusing on their antioxidant, cardioprotective, anticancer, anti-inflammatory, anti-aging, and antimicrobial properties. Grapes are rich in polyphenols, including anthocyanins, flavonoids, resveratrol, and phenolic acids, which are found mainly in the skin, seeds, and stems. These compounds have been extensively studied for their health benefits, including reducing oxidative stress, preventing cardiovascular diseases, and inhibiting cancer development. The review also discusses the extraction, isolation, and identification methods of grape polyphenols, as well as their bioavailability and potential toxicity. The bioavailability of polyphenols varies, with some high molecular weight compounds not being absorbed. While grape polyphenols show promising health benefits, higher concentrations may have negative effects. The review highlights the importance of further research to understand the mechanisms of action and optimize the use of grape polyphenols in functional foods and pharmaceuticals. The potential toxicity of some polyphenols, such as epicatechin, is also discussed. Overall, grape polyphenols have significant potential in disease prevention and health promotion.This review summarizes the biological activities of polyphenols from grapes, focusing on their antioxidant, cardioprotective, anticancer, anti-inflammatory, anti-aging, and antimicrobial properties. Grapes are rich in polyphenols, including anthocyanins, flavonoids, resveratrol, and phenolic acids, which are found mainly in the skin, seeds, and stems. These compounds have been extensively studied for their health benefits, including reducing oxidative stress, preventing cardiovascular diseases, and inhibiting cancer development. The review also discusses the extraction, isolation, and identification methods of grape polyphenols, as well as their bioavailability and potential toxicity. The bioavailability of polyphenols varies, with some high molecular weight compounds not being absorbed. While grape polyphenols show promising health benefits, higher concentrations may have negative effects. The review highlights the importance of further research to understand the mechanisms of action and optimize the use of grape polyphenols in functional foods and pharmaceuticals. The potential toxicity of some polyphenols, such as epicatechin, is also discussed. Overall, grape polyphenols have significant potential in disease prevention and health promotion.