Biologically active secondary metabolites from white-rot fungi have significant potential in various sectors, including pharmaceuticals, biotechnology, and medicine. These metabolites are produced by white-rot fungi, which are capable of decomposing all components of plant cell walls, playing a crucial role in carbon and nitrogen cycles. They produce a variety of bioactive substances, including antimicrobial and anticancer compounds, which are of interest for pharmaceutical industries. This review highlights the secondary metabolites produced by white-rot fungi with various bioactivities, emphasizing their potential applications. White-rot fungi are known for their ability to produce secondary metabolites that exhibit diverse biological functions, such as antioxidant, antimicrobial, anti-inflammatory, antifungal, and antiviral properties. These metabolites are derived from primary metabolic pathways and are not essential for the growth and development of the organism. They play a significant role in ecological dynamics by increasing tolerance to environmental stresses and extreme conditions. The biosynthesis of fungal secondary metabolites is typically based on the mevalonic acid pathway, the acetate pathway, and carbohydrate/polysaccharide synthesis. These metabolites can be divided into high and low molecular weight compounds. High molecular weight compounds include polysaccharides and enzymes, while low molecular weight compounds include terpenoids, phenols, and indoles. Among these, approximately 47% of the bioactive compounds originate from fungal strains. Several studies have shown that white-rot fungi, such as Schizophyllum commune, produce bioactive secondary metabolites with potential therapeutic applications. For example, schizostatin, a metabolite from S. commune, has been shown to inhibit cholesterol synthesis and exhibit antimicrobial activity. Additionally, S. commune extract has been proposed as a potential biocontrol agent due to its antifungal and disease-control properties. Other white-rot fungi, such as Pleurotus sajor-caju, have been found to produce secondary metabolites with antimicrobial, anticancer, antipyretic, astringent, and antiviral properties. These metabolites, including phenols, flavonoids, tannins, and alkaloids, have significant potential for commercial and pharmaceutical applications. The review also highlights the importance of further research to understand the mechanisms of action of these bioactive compounds and their potential applications in various fields. The study emphasizes the need for more research to explore the potential of these metabolites in the development of new drugs and therapeutic agents.Biologically active secondary metabolites from white-rot fungi have significant potential in various sectors, including pharmaceuticals, biotechnology, and medicine. These metabolites are produced by white-rot fungi, which are capable of decomposing all components of plant cell walls, playing a crucial role in carbon and nitrogen cycles. They produce a variety of bioactive substances, including antimicrobial and anticancer compounds, which are of interest for pharmaceutical industries. This review highlights the secondary metabolites produced by white-rot fungi with various bioactivities, emphasizing their potential applications. White-rot fungi are known for their ability to produce secondary metabolites that exhibit diverse biological functions, such as antioxidant, antimicrobial, anti-inflammatory, antifungal, and antiviral properties. These metabolites are derived from primary metabolic pathways and are not essential for the growth and development of the organism. They play a significant role in ecological dynamics by increasing tolerance to environmental stresses and extreme conditions. The biosynthesis of fungal secondary metabolites is typically based on the mevalonic acid pathway, the acetate pathway, and carbohydrate/polysaccharide synthesis. These metabolites can be divided into high and low molecular weight compounds. High molecular weight compounds include polysaccharides and enzymes, while low molecular weight compounds include terpenoids, phenols, and indoles. Among these, approximately 47% of the bioactive compounds originate from fungal strains. Several studies have shown that white-rot fungi, such as Schizophyllum commune, produce bioactive secondary metabolites with potential therapeutic applications. For example, schizostatin, a metabolite from S. commune, has been shown to inhibit cholesterol synthesis and exhibit antimicrobial activity. Additionally, S. commune extract has been proposed as a potential biocontrol agent due to its antifungal and disease-control properties. Other white-rot fungi, such as Pleurotus sajor-caju, have been found to produce secondary metabolites with antimicrobial, anticancer, antipyretic, astringent, and antiviral properties. These metabolites, including phenols, flavonoids, tannins, and alkaloids, have significant potential for commercial and pharmaceutical applications. The review also highlights the importance of further research to understand the mechanisms of action of these bioactive compounds and their potential applications in various fields. The study emphasizes the need for more research to explore the potential of these metabolites in the development of new drugs and therapeutic agents.