The article provides an overview of Biopython, a freely available open-source Python library for computational molecular biology and bioinformatics. Biopython offers a wide range of modules for tasks such as reading and writing sequence files, handling 3D macromolecular structures, interacting with tools like BLAST, ClustalW, and EMBOSS, accessing online databases, and performing statistical learning. The core sequence representation is the Seq object, which can be annotated using SeqRecord objects. Biopython also includes modules for sequence alignment, phylogenetic analysis, and population genetics. The article highlights the project's features, availability, and community support, emphasizing its role in both software development and everyday bioinformatics tasks.The article provides an overview of Biopython, a freely available open-source Python library for computational molecular biology and bioinformatics. Biopython offers a wide range of modules for tasks such as reading and writing sequence files, handling 3D macromolecular structures, interacting with tools like BLAST, ClustalW, and EMBOSS, accessing online databases, and performing statistical learning. The core sequence representation is the Seq object, which can be annotated using SeqRecord objects. Biopython also includes modules for sequence alignment, phylogenetic analysis, and population genetics. The article highlights the project's features, availability, and community support, emphasizing its role in both software development and everyday bioinformatics tasks.