This review explores the applications of molecularly imprinted polymer (MIP)-based nanomaterials in biosensing. MIPs are synthetic polymers with specific binding sites for target molecules, offering high selectivity, sensitivity, and reusability. The review discusses various sensor technologies, including electrochemical, piezoelectric, and optical sensors, and highlights the advantages of MIP-based sensors in detecting a wide range of molecules. The preparation methods of MIPs, such as molecular imprinting, are detailed, emphasizing their role in creating tailored recognition sites. The review also covers recent advancements in MIP-based sensors, including imprinted-particle and nanogel-based sensors, which have shown promising results in applications like food safety, environmental monitoring, and medical diagnostics. The integration of MIPs with nanotechnology has led to the development of advanced sensors with enhanced performance, stability, and functionality. The review emphasizes the potential of MIP-based sensors in revolutionizing detection platforms across various fields, while also addressing challenges in achieving higher accuracy and reliability. Overall, the review provides a comprehensive overview of the current state of MIP-based sensor technology and its future prospects in biosensing applications.This review explores the applications of molecularly imprinted polymer (MIP)-based nanomaterials in biosensing. MIPs are synthetic polymers with specific binding sites for target molecules, offering high selectivity, sensitivity, and reusability. The review discusses various sensor technologies, including electrochemical, piezoelectric, and optical sensors, and highlights the advantages of MIP-based sensors in detecting a wide range of molecules. The preparation methods of MIPs, such as molecular imprinting, are detailed, emphasizing their role in creating tailored recognition sites. The review also covers recent advancements in MIP-based sensors, including imprinted-particle and nanogel-based sensors, which have shown promising results in applications like food safety, environmental monitoring, and medical diagnostics. The integration of MIPs with nanotechnology has led to the development of advanced sensors with enhanced performance, stability, and functionality. The review emphasizes the potential of MIP-based sensors in revolutionizing detection platforms across various fields, while also addressing challenges in achieving higher accuracy and reliability. Overall, the review provides a comprehensive overview of the current state of MIP-based sensor technology and its future prospects in biosensing applications.