The study investigates the neuronal correlates of tone and color processing in the prefrontal cortex. Neurons were selected based on stable intertrial baseline and significant differences in firing rates between high-tone and low-tone stimuli. Four trial periods were analyzed: the first 1 second of the tone, the 10-second delay, the first 1 second of color presentation, and 400 ms of color presentation. Four delta values (Δ1 to Δ4) were determined to measure differences in firing responses to tones, delays, colors, and chosen colors. The correlation of these delta values was analyzed using bootstrapping, showing weak correlations between tone and color-related differences. The study also explores the interaction between the transcription factors Empty Spiracles (EMS) and Buttonhead (BTD) in Drosophila head segmentation, demonstrating that EMS can escape phenotypic suppression by BTD and cause homeotic transformations in the head region.The study investigates the neuronal correlates of tone and color processing in the prefrontal cortex. Neurons were selected based on stable intertrial baseline and significant differences in firing rates between high-tone and low-tone stimuli. Four trial periods were analyzed: the first 1 second of the tone, the 10-second delay, the first 1 second of color presentation, and 400 ms of color presentation. Four delta values (Δ1 to Δ4) were determined to measure differences in firing responses to tones, delays, colors, and chosen colors. The correlation of these delta values was analyzed using bootstrapping, showing weak correlations between tone and color-related differences. The study also explores the interaction between the transcription factors Empty Spiracles (EMS) and Buttonhead (BTD) in Drosophila head segmentation, demonstrating that EMS can escape phenotypic suppression by BTD and cause homeotic transformations in the head region.