This review discusses the development and enhancement of enzymatic activity in enzyme-metal–organic framework (MOF) composites. Enzymes, as highly efficient biocatalysts, are vulnerable to denaturation due to their sensitivity to temperature, pH, and organic solvents. Encapsulation within MOFs offers protection but often reduces enzyme activity. Recent advancements have focused on improving enzymatic activity in MOF composites through various strategies, including modifying enzyme structures, tuning MOF properties, optimizing enzyme-MOF interactions, and facilitating substrate/product exchange. Modifying enzyme structures, such as engineering active sites or using biochemical modifications, has shown promise in enhancing activity. Tuning MOF properties, like hydrophobicity, can also improve enzyme activity by altering the microenvironment. Optimizing interactions between enzymes and MOFs, such as through pre-nucleation of metal ions or hydrophilic/hydrophobic interactions, enhances stability and catalytic performance. Facilitating substrate and product exchange through pore size modulation or defect engineering improves reaction efficiency. These strategies have led to the development of highly active enzyme-MOF composites with applications in catalysis, biosensing, biomedicine, and environmental remediation. The review highlights the potential of enzyme-MOF composites for practical applications, emphasizing the need for further research to address challenges such as cost, scalability, and quality control. Future perspectives include the development of scalable fabrication methods and exploration of new applications for these composites.This review discusses the development and enhancement of enzymatic activity in enzyme-metal–organic framework (MOF) composites. Enzymes, as highly efficient biocatalysts, are vulnerable to denaturation due to their sensitivity to temperature, pH, and organic solvents. Encapsulation within MOFs offers protection but often reduces enzyme activity. Recent advancements have focused on improving enzymatic activity in MOF composites through various strategies, including modifying enzyme structures, tuning MOF properties, optimizing enzyme-MOF interactions, and facilitating substrate/product exchange. Modifying enzyme structures, such as engineering active sites or using biochemical modifications, has shown promise in enhancing activity. Tuning MOF properties, like hydrophobicity, can also improve enzyme activity by altering the microenvironment. Optimizing interactions between enzymes and MOFs, such as through pre-nucleation of metal ions or hydrophilic/hydrophobic interactions, enhances stability and catalytic performance. Facilitating substrate and product exchange through pore size modulation or defect engineering improves reaction efficiency. These strategies have led to the development of highly active enzyme-MOF composites with applications in catalysis, biosensing, biomedicine, and environmental remediation. The review highlights the potential of enzyme-MOF composites for practical applications, emphasizing the need for further research to address challenges such as cost, scalability, and quality control. Future perspectives include the development of scalable fabrication methods and exploration of new applications for these composites.