This review discusses recent advancements in enhancing the enzymatic activity of enzyme-metal-organic framework (MOF) composites. Enzymes, as efficient biocatalysts, face challenges such as low thermal stability, limited pH tolerance, and sensitivity to organic solvents. Encapsulation in MOFs protects enzymes but often reduces activity. Recent research has developed enzyme-MOF composites that maintain or enhance catalytic performance. Strategies include modifying enzyme structures, tuning MOF properties, optimizing enzyme-MOF interactions, and facilitating substrate/product exchange. Techniques like ultrasound treatment and biomimetic mineralization have shown promise in improving enzyme stability and activity. The review highlights the potential of enzyme-MOF composites in various applications, including catalysis, biosensing, and biomedicine. Challenges remain in scaling up production and ensuring cost-effectiveness. Future research aims to further enhance enzyme activity and explore new applications for these composites.This review discusses recent advancements in enhancing the enzymatic activity of enzyme-metal-organic framework (MOF) composites. Enzymes, as efficient biocatalysts, face challenges such as low thermal stability, limited pH tolerance, and sensitivity to organic solvents. Encapsulation in MOFs protects enzymes but often reduces activity. Recent research has developed enzyme-MOF composites that maintain or enhance catalytic performance. Strategies include modifying enzyme structures, tuning MOF properties, optimizing enzyme-MOF interactions, and facilitating substrate/product exchange. Techniques like ultrasound treatment and biomimetic mineralization have shown promise in improving enzyme stability and activity. The review highlights the potential of enzyme-MOF composites in various applications, including catalysis, biosensing, and biomedicine. Challenges remain in scaling up production and ensuring cost-effectiveness. Future research aims to further enhance enzyme activity and explore new applications for these composites.