The study aimed to investigate the acute effects of uninterrupted sitting versus sitting interrupted by brief bouts of light- or moderate-intensity walking on postprandial glucose and insulin levels in overweight/obese adults. Overweight/obese adults (n=19) participated in a randomized, three-period, three-treatment acute crossover trial. The results showed that both light- and moderate-intensity activity breaks significantly reduced postprandial glucose and insulin responses compared to uninterrupted sitting. Specifically, the glucose incremental area under the curve (iAUC) was 24.1% lower after light-intensity activity breaks and 29.6% lower after moderate-intensity activity breaks compared to uninterrupted sitting. The insulin iAUC was also reduced by 23% after both activity-break conditions. These findings suggest that brief interruptions to prolonged sitting can improve glucose metabolism and may have important public health and clinical implications for reducing cardiovascular risk.The study aimed to investigate the acute effects of uninterrupted sitting versus sitting interrupted by brief bouts of light- or moderate-intensity walking on postprandial glucose and insulin levels in overweight/obese adults. Overweight/obese adults (n=19) participated in a randomized, three-period, three-treatment acute crossover trial. The results showed that both light- and moderate-intensity activity breaks significantly reduced postprandial glucose and insulin responses compared to uninterrupted sitting. Specifically, the glucose incremental area under the curve (iAUC) was 24.1% lower after light-intensity activity breaks and 29.6% lower after moderate-intensity activity breaks compared to uninterrupted sitting. The insulin iAUC was also reduced by 23% after both activity-break conditions. These findings suggest that brief interruptions to prolonged sitting can improve glucose metabolism and may have important public health and clinical implications for reducing cardiovascular risk.