Breaking up prolonged sitting reduces postprandial glucose and insulin responses in overweight and obese adults. A randomized crossover trial with 19 participants aged 45–65 years found that interrupting sitting with 2-minute bouts of light or moderate-intensity walking significantly reduced the area under the curve (iAUC) for glucose and insulin compared to uninterrupted sitting. The glucose iAUC was 5.2 mmol/L·h for light-intensity walking and 4.9 mmol/L·h for moderate-intensity walking, versus 6.9 mmol/L·h for uninterrupted sitting. Similarly, insulin iAUC was reduced to 633.6 pmol/L·h and 637.6 pmol/L·h for light and moderate-intensity walking, respectively, compared to 828.6 pmol/L·h for uninterrupted sitting. These findings suggest that brief activity breaks during prolonged sitting can improve glucose metabolism and potentially reduce cardiovascular risk. Prolonged sitting is associated with increased cardiovascular and all-cause mortality, independent of physical activity and adiposity. Observational studies show that frequent interruptions to sedentary time are beneficial, while prolonged sitting is detrimental. The study also found that regular moderate-intensity exercise improves postprandial glucose and insulin levels, and that light-intensity activity can also reduce postprandial glucose responses. These findings support the hypothesis that brief interruptions to sedentary time with light-intensity activity can reduce postprandial glucose and insulin responses during prolonged sitting. The study's results indicate that interrupting prolonged sitting with short bouts of light or moderate-intensity walking can lower postprandial glucose and insulin levels, which may improve glucose metabolism and reduce cardiovascular risk. The findings suggest that such interventions could be an important public health and clinical strategy for reducing cardiovascular risk. However, further research is needed to determine the long-term effects of different sitting-time patterns and to explore the metabolic mechanisms underlying these effects. The study also highlights the importance of regular physical activity in reducing cardiovascular risk and the potential benefits of brief activity breaks during prolonged sitting.Breaking up prolonged sitting reduces postprandial glucose and insulin responses in overweight and obese adults. A randomized crossover trial with 19 participants aged 45–65 years found that interrupting sitting with 2-minute bouts of light or moderate-intensity walking significantly reduced the area under the curve (iAUC) for glucose and insulin compared to uninterrupted sitting. The glucose iAUC was 5.2 mmol/L·h for light-intensity walking and 4.9 mmol/L·h for moderate-intensity walking, versus 6.9 mmol/L·h for uninterrupted sitting. Similarly, insulin iAUC was reduced to 633.6 pmol/L·h and 637.6 pmol/L·h for light and moderate-intensity walking, respectively, compared to 828.6 pmol/L·h for uninterrupted sitting. These findings suggest that brief activity breaks during prolonged sitting can improve glucose metabolism and potentially reduce cardiovascular risk. Prolonged sitting is associated with increased cardiovascular and all-cause mortality, independent of physical activity and adiposity. Observational studies show that frequent interruptions to sedentary time are beneficial, while prolonged sitting is detrimental. The study also found that regular moderate-intensity exercise improves postprandial glucose and insulin levels, and that light-intensity activity can also reduce postprandial glucose responses. These findings support the hypothesis that brief interruptions to sedentary time with light-intensity activity can reduce postprandial glucose and insulin responses during prolonged sitting. The study's results indicate that interrupting prolonged sitting with short bouts of light or moderate-intensity walking can lower postprandial glucose and insulin levels, which may improve glucose metabolism and reduce cardiovascular risk. The findings suggest that such interventions could be an important public health and clinical strategy for reducing cardiovascular risk. However, further research is needed to determine the long-term effects of different sitting-time patterns and to explore the metabolic mechanisms underlying these effects. The study also highlights the importance of regular physical activity in reducing cardiovascular risk and the potential benefits of brief activity breaks during prolonged sitting.