This study presents an innovative feedback-regulation system for highly sensitive sulfur dioxide (SO₂) sensing using Pt sites on MoS₂ supports. The system is designed by modifying the interaction between single Pt atoms and adjacent sulfur species, which enhances the electron transfer path and improves SO₂ sensing performance. The Pt₁-MoS₂-def sensors exhibit high sensitivity and a low detection limit (3.14% to 500 ppb SO₂) at room temperature. The system enables real-time monitoring of SO₂ levels for plant growth, with wireless data transmission and cloud storage capabilities. The feedback-regulation system is confirmed through in situ Raman, ex situ XPS, and DFT analysis, showing that the system expands the electron transfer path from single Pt sites to the entire Pt-MoS₂ support in SO₂ gas. The study also demonstrates that the system enhances SO₂ adsorption and electronic transformation, leading to improved sensing performance. The Pt₁-MoS₂-def sensors show superior performance compared to other sensors, with high selectivity and stability. The study highlights the importance of atomic interface design in gas sensing and provides insights into the mechanism of SO₂ sensing. The results suggest that the feedback-regulation system is crucial for developing high-performance gas sensors with low detection limits and high sensitivity. The study also discusses the application of the sensors in plant growth monitoring, emphasizing the importance of real-time SO₂ monitoring for optimizing plant growth. The research contributes to the development of efficient and reliable gas sensors for environmental and agricultural applications.This study presents an innovative feedback-regulation system for highly sensitive sulfur dioxide (SO₂) sensing using Pt sites on MoS₂ supports. The system is designed by modifying the interaction between single Pt atoms and adjacent sulfur species, which enhances the electron transfer path and improves SO₂ sensing performance. The Pt₁-MoS₂-def sensors exhibit high sensitivity and a low detection limit (3.14% to 500 ppb SO₂) at room temperature. The system enables real-time monitoring of SO₂ levels for plant growth, with wireless data transmission and cloud storage capabilities. The feedback-regulation system is confirmed through in situ Raman, ex situ XPS, and DFT analysis, showing that the system expands the electron transfer path from single Pt sites to the entire Pt-MoS₂ support in SO₂ gas. The study also demonstrates that the system enhances SO₂ adsorption and electronic transformation, leading to improved sensing performance. The Pt₁-MoS₂-def sensors show superior performance compared to other sensors, with high selectivity and stability. The study highlights the importance of atomic interface design in gas sensing and provides insights into the mechanism of SO₂ sensing. The results suggest that the feedback-regulation system is crucial for developing high-performance gas sensors with low detection limits and high sensitivity. The study also discusses the application of the sensors in plant growth monitoring, emphasizing the importance of real-time SO₂ monitoring for optimizing plant growth. The research contributes to the development of efficient and reliable gas sensors for environmental and agricultural applications.