This review discusses the potential of CAR-NK (Chimeric Antigen Receptor Natural Killer) cells in cancer therapy, highlighting their advantages over CAR-T cells and the challenges they face. CAR-NK cells are safer and more versatile, with fewer side effects and a wider range of clinical applications. The review covers the selection of targets, structure construction, and combination therapies for CAR-NK cells, emphasizing the importance of overcoming the inhibitory tumor microenvironment. Key strategies include combining CAR-NK cells with anti-PD-1/PD-L1, radiotherapy, chemotherapy, kinase inhibitors, proteasome inhibitors, STING agonists, oncolytic viruses, and photothermal therapy. These combinations aim to enhance the proliferation, migration, and cytotoxicity of NK cells, improving their efficacy in treating both hematological and solid tumors. The review also discusses the current research status, existing problems, and future prospects of CAR-NK cell therapy, providing insights for further development and clinical application.This review discusses the potential of CAR-NK (Chimeric Antigen Receptor Natural Killer) cells in cancer therapy, highlighting their advantages over CAR-T cells and the challenges they face. CAR-NK cells are safer and more versatile, with fewer side effects and a wider range of clinical applications. The review covers the selection of targets, structure construction, and combination therapies for CAR-NK cells, emphasizing the importance of overcoming the inhibitory tumor microenvironment. Key strategies include combining CAR-NK cells with anti-PD-1/PD-L1, radiotherapy, chemotherapy, kinase inhibitors, proteasome inhibitors, STING agonists, oncolytic viruses, and photothermal therapy. These combinations aim to enhance the proliferation, migration, and cytotoxicity of NK cells, improving their efficacy in treating both hematological and solid tumors. The review also discusses the current research status, existing problems, and future prospects of CAR-NK cell therapy, providing insights for further development and clinical application.