The article introduces CHOPCHOP v3, an updated web tool for identifying CRISPR-Cas single guide RNA (sgRNA) targets. This major update expands the tool's capabilities beyond genome editing to include RNA targeting with Cas13, new DNA targeting modes such as CRISPR activation/repression and targeted enrichment for long-read sequencing, and improved visualization of results to aid in avoiding truncated protein expression. CHOPCHOP v3 supports over 200 genomes and includes a command-line script for handling larger jobs and unsupported genomes. The tool now offers a user-friendly interface with advanced options and a queuing system to manage heavy traffic. Key improvements include RNA accessibility predictions, support for alternative transcript isoforms, and enhanced visualization of in-frame downstream ATGs. The article also discusses the broader implications of these updates in the context of CRISPR-Cas technology and its diverse applications.The article introduces CHOPCHOP v3, an updated web tool for identifying CRISPR-Cas single guide RNA (sgRNA) targets. This major update expands the tool's capabilities beyond genome editing to include RNA targeting with Cas13, new DNA targeting modes such as CRISPR activation/repression and targeted enrichment for long-read sequencing, and improved visualization of results to aid in avoiding truncated protein expression. CHOPCHOP v3 supports over 200 genomes and includes a command-line script for handling larger jobs and unsupported genomes. The tool now offers a user-friendly interface with advanced options and a queuing system to manage heavy traffic. Key improvements include RNA accessibility predictions, support for alternative transcript isoforms, and enhanced visualization of in-frame downstream ATGs. The article also discusses the broader implications of these updates in the context of CRISPR-Cas technology and its diverse applications.