Candida auris, an emerging human fungal pathogen first identified in Japan in 2009 and detected in the U.S. in 2016, has been found in the oral cavity of a dog in Kansas for the first time. This study reports the first identification of C. auris in a non-human mammal in the U.S. and the first isolate from Kansas. The dog, a 2-year-old Retriever mix, was found to have four fungal species, including C. auris. The isolate belongs to Clade IV, which has been found in Chicago and Florida. It is resistant to fluconazole, terbinafine, and amphotericin B but susceptible to caspofungin. The source of the colonization is unknown, and there is no evidence of transmission to humans or other dogs. The presence of C. auris in a pet raises concerns about zoonotic transmission. The study highlights the potential for C. auris to become a new source of zoonotic spread and for antifungal resistance. The isolate was analyzed using molecular techniques, and its genome was sequenced. The findings suggest that C. auris may be present in Kansas as a potential reservoir despite no human infections being reported. The study emphasizes the need for vigilance among veterinary and human healthcare professionals in detecting and responding to C. auris cases.Candida auris, an emerging human fungal pathogen first identified in Japan in 2009 and detected in the U.S. in 2016, has been found in the oral cavity of a dog in Kansas for the first time. This study reports the first identification of C. auris in a non-human mammal in the U.S. and the first isolate from Kansas. The dog, a 2-year-old Retriever mix, was found to have four fungal species, including C. auris. The isolate belongs to Clade IV, which has been found in Chicago and Florida. It is resistant to fluconazole, terbinafine, and amphotericin B but susceptible to caspofungin. The source of the colonization is unknown, and there is no evidence of transmission to humans or other dogs. The presence of C. auris in a pet raises concerns about zoonotic transmission. The study highlights the potential for C. auris to become a new source of zoonotic spread and for antifungal resistance. The isolate was analyzed using molecular techniques, and its genome was sequenced. The findings suggest that C. auris may be present in Kansas as a potential reservoir despite no human infections being reported. The study emphasizes the need for vigilance among veterinary and human healthcare professionals in detecting and responding to C. auris cases.