This study investigates the distinct roles of caspase-9, caspase-3, and caspase-7 during intrinsic apoptosis. The research reveals that caspase-9 is essential for mitochondrial remodeling and ROS production by cleaving Bid into tBid, which is necessary for mitochondrial changes and ROS generation. Caspase-3 inhibits ROS production and is crucial for efficient apoptosis execution. Caspase-7 is involved in cell detachment from the extracellular matrix but does not affect sensitivity to intrinsic apoptosis. These findings suggest that each caspase has a unique function in the apoptotic process, with caspase-9 initiating mitochondrial changes, caspase-3 limiting ROS production, and caspase-7 facilitating cell detachment. The study highlights the importance of these caspases in different stages of apoptosis and their distinct contributions to the overall process.This study investigates the distinct roles of caspase-9, caspase-3, and caspase-7 during intrinsic apoptosis. The research reveals that caspase-9 is essential for mitochondrial remodeling and ROS production by cleaving Bid into tBid, which is necessary for mitochondrial changes and ROS generation. Caspase-3 inhibits ROS production and is crucial for efficient apoptosis execution. Caspase-7 is involved in cell detachment from the extracellular matrix but does not affect sensitivity to intrinsic apoptosis. These findings suggest that each caspase has a unique function in the apoptotic process, with caspase-9 initiating mitochondrial changes, caspase-3 limiting ROS production, and caspase-7 facilitating cell detachment. The study highlights the importance of these caspases in different stages of apoptosis and their distinct contributions to the overall process.