The study investigates the distinct roles of caspase-9, caspase-3, and caspase-7 during intrinsic apoptosis. Caspase-9 is shown to be essential for mitochondrial remodeling and ROS production by cleaving Bid into tBid. Caspase-3 inhibits ROS production and is crucial for efficient apoptosis execution, while caspase-7 is involved in cell detachment from the extracellular matrix (ECM). The findings suggest that these caspases have specific functions in the intrinsic apoptosis pathway, with caspase-9 remodeling mitochondria, caspase-3 regulating ROS, and caspase-7 facilitating cell detachment.The study investigates the distinct roles of caspase-9, caspase-3, and caspase-7 during intrinsic apoptosis. Caspase-9 is shown to be essential for mitochondrial remodeling and ROS production by cleaving Bid into tBid. Caspase-3 inhibits ROS production and is crucial for efficient apoptosis execution, while caspase-7 is involved in cell detachment from the extracellular matrix (ECM). The findings suggest that these caspases have specific functions in the intrinsic apoptosis pathway, with caspase-9 remodeling mitochondria, caspase-3 regulating ROS, and caspase-7 facilitating cell detachment.