This study explores the use of gelatin methacrylate (GelMA) as a cost-effective, cell-responsive hydrogel platform for creating cell-laden microtissues and microfluidic devices. GelMA, a photopolymerizable hydrogel derived from modified natural extracellular matrix (ECM) components, was found to have high pattern fidelity and resolution, making it suitable for creating perfusable microfluidic channels. Cells, including human umbilical vein endothelial cells (HUVEC) and NIH 3T3 fibroblasts, readily bound to, proliferated, elongated, and migrated on GelMA substrates and within encapsulated GelMA micropatterns. The mechanical properties and swelling characteristics of GelMA were tunable by adjusting the degree of methacrylation and gel concentration, respectively. These findings suggest that GelMA could be useful for creating complex, cell-responsive microtissues, such as endothelialized microvasculature, and for applications requiring cell-responsive microengineered hydrogels.This study explores the use of gelatin methacrylate (GelMA) as a cost-effective, cell-responsive hydrogel platform for creating cell-laden microtissues and microfluidic devices. GelMA, a photopolymerizable hydrogel derived from modified natural extracellular matrix (ECM) components, was found to have high pattern fidelity and resolution, making it suitable for creating perfusable microfluidic channels. Cells, including human umbilical vein endothelial cells (HUVEC) and NIH 3T3 fibroblasts, readily bound to, proliferated, elongated, and migrated on GelMA substrates and within encapsulated GelMA micropatterns. The mechanical properties and swelling characteristics of GelMA were tunable by adjusting the degree of methacrylation and gel concentration, respectively. These findings suggest that GelMA could be useful for creating complex, cell-responsive microtissues, such as endothelialized microvasculature, and for applications requiring cell-responsive microengineered hydrogels.