This review explores the relationship between spermatogonial stem cells (SSCs) and the JAK/STAT signaling pathway. SSCs, found in the testes of male mammals, are essential for tissue regeneration, immunomodulation, and regenerative medicine. The JAK/STAT pathway significantly influences SSC proliferation, maturation, and differentiation, affecting their immunomodulatory, tissue differentiation, homing, and adaptation to the microenvironment. The review discusses the role of the JAK/STAT pathway in SSCs and their target cells, highlighting its implications for preclinical research and cellular engineering. Despite advancements, research in humans and larger mammals remains limited, necessitating further investigation to establish a comprehensive theoretical framework. The review also examines the JAK/STAT pathway's role in SSC proliferation, tissue differentiation, immune regulation, and homing migration. It emphasizes the importance of understanding the JAK/STAT pathway in SSCs for future research and clinical applications. The study highlights the need for a comprehensive theoretical framework for SSCs across different species. The review concludes that the JAK/STAT pathway plays a crucial role in regulating SSCs and their target cells, with implications for disease treatment and adjuvant therapy. The study underscores the importance of further research on SSCs and the JAK/STAT pathway for advancing regenerative medicine and stem cell therapies.This review explores the relationship between spermatogonial stem cells (SSCs) and the JAK/STAT signaling pathway. SSCs, found in the testes of male mammals, are essential for tissue regeneration, immunomodulation, and regenerative medicine. The JAK/STAT pathway significantly influences SSC proliferation, maturation, and differentiation, affecting their immunomodulatory, tissue differentiation, homing, and adaptation to the microenvironment. The review discusses the role of the JAK/STAT pathway in SSCs and their target cells, highlighting its implications for preclinical research and cellular engineering. Despite advancements, research in humans and larger mammals remains limited, necessitating further investigation to establish a comprehensive theoretical framework. The review also examines the JAK/STAT pathway's role in SSC proliferation, tissue differentiation, immune regulation, and homing migration. It emphasizes the importance of understanding the JAK/STAT pathway in SSCs for future research and clinical applications. The study highlights the need for a comprehensive theoretical framework for SSCs across different species. The review concludes that the JAK/STAT pathway plays a crucial role in regulating SSCs and their target cells, with implications for disease treatment and adjuvant therapy. The study underscores the importance of further research on SSCs and the JAK/STAT pathway for advancing regenerative medicine and stem cell therapies.