ChIP-seq accurately predicts tissue-specific activity of enhancers. Researchers used chromatin immunoprecipitation with the enhancer-associated protein p300 followed by massively parallel sequencing to map p300 binding sites in mouse embryonic forebrain, midbrain, and limb tissues. They tested 86 sequences in transgenic mice and found that p300 binding sites predicted enhancer activity in the tissues where they were active. The results show that in vivo mapping of p300 binding is a highly accurate method for identifying enhancers and their associated activities. This data can be used to study the role of tissue-specific enhancers in human biology and disease on a genome-wide scale. The study found that most p300-bound regions are conserved, suggesting that p300 peaks are a highly accurate predictor of in vivo enhancers and their spatial activity patterns. The correlation between p300-enriched regions and gene expression patterns was also examined, and it was found that p300 peaks are enriched near genes that are expressed in the same tissue. This indicates that p300 binding is closely associated with the regulation of gene expression in specific tissues. The study also compared the accuracy of p300 binding in predicting enhancer activity with previous methods based on evolutionary conservation. The results showed that p300 binding has a much higher accuracy in predicting enhancer activity than evolutionary conservation-based methods. This suggests that p300 binding is a more reliable method for identifying enhancers and their activity patterns. The study provides a new approach for locating enhancers in the human genome with higher accuracy than conservation-based methods. It also predicts the in vivo activity patterns of enhancers with higher accuracy than motif-based computational methods. The results indicate that p300 peaks are a highly accurate predictor of in vivo enhancers and their spatial activity patterns. This study highlights the importance of p300 in the regulation of gene expression and provides a valuable tool for studying the role of tissue-specific enhancers in human biology and disease.ChIP-seq accurately predicts tissue-specific activity of enhancers. Researchers used chromatin immunoprecipitation with the enhancer-associated protein p300 followed by massively parallel sequencing to map p300 binding sites in mouse embryonic forebrain, midbrain, and limb tissues. They tested 86 sequences in transgenic mice and found that p300 binding sites predicted enhancer activity in the tissues where they were active. The results show that in vivo mapping of p300 binding is a highly accurate method for identifying enhancers and their associated activities. This data can be used to study the role of tissue-specific enhancers in human biology and disease on a genome-wide scale. The study found that most p300-bound regions are conserved, suggesting that p300 peaks are a highly accurate predictor of in vivo enhancers and their spatial activity patterns. The correlation between p300-enriched regions and gene expression patterns was also examined, and it was found that p300 peaks are enriched near genes that are expressed in the same tissue. This indicates that p300 binding is closely associated with the regulation of gene expression in specific tissues. The study also compared the accuracy of p300 binding in predicting enhancer activity with previous methods based on evolutionary conservation. The results showed that p300 binding has a much higher accuracy in predicting enhancer activity than evolutionary conservation-based methods. This suggests that p300 binding is a more reliable method for identifying enhancers and their activity patterns. The study provides a new approach for locating enhancers in the human genome with higher accuracy than conservation-based methods. It also predicts the in vivo activity patterns of enhancers with higher accuracy than motif-based computational methods. The results indicate that p300 peaks are a highly accurate predictor of in vivo enhancers and their spatial activity patterns. This study highlights the importance of p300 in the regulation of gene expression and provides a valuable tool for studying the role of tissue-specific enhancers in human biology and disease.