This study characterized the oral fungal microbiome (mycobiome) in 20 healthy individuals using a novel multitag pyrosequencing approach with pan-fungal internal transcribed spacer (ITS) primers. The results revealed a diverse fungal community, with 74 culturable and 11 non-culturable fungal genera identified. *Candida* species were the most prevalent, followed by *Cladosporium*, *Aureobasidium*, *Saccharomycetales*, *Aspergillus*, *Fusarium*, and *Cryptococcus*. The presence of these fungi varied among individuals, with 15 genera present in at least 20% of the samples. The study also found that White males and Asian males clustered differently from each other, while both Asian and White females clustered together. This is the first study to identify the "basal mycobiome" of healthy individuals, providing a foundation for further research on the oral mycobiome in health and disease.This study characterized the oral fungal microbiome (mycobiome) in 20 healthy individuals using a novel multitag pyrosequencing approach with pan-fungal internal transcribed spacer (ITS) primers. The results revealed a diverse fungal community, with 74 culturable and 11 non-culturable fungal genera identified. *Candida* species were the most prevalent, followed by *Cladosporium*, *Aureobasidium*, *Saccharomycetales*, *Aspergillus*, *Fusarium*, and *Cryptococcus*. The presence of these fungi varied among individuals, with 15 genera present in at least 20% of the samples. The study also found that White males and Asian males clustered differently from each other, while both Asian and White females clustered together. This is the first study to identify the "basal mycobiome" of healthy individuals, providing a foundation for further research on the oral mycobiome in health and disease.