A study analyzed the chemical composition of 29 industrial hempseed (Cannabis sativa L.) varieties, focusing on fatty acid profiles, amino acid content, mineral composition, and cannabinoid levels. The results showed significant variations among the varieties for all measured parameters. Proximate composition revealed that crude protein and fat contents ranged from 21.6–28.9% and 21.1–35.7%, respectively. Fatty acids included linoleic acid (52.79–57.13%), α-linolenic acid (12.62–20.24%), and oleic acid (11.08–17.81%). All essential amino acids were present, with arginine being abundant and lysine limiting. Mineral content varied, with potassium and iron as the main macro- and microminerals. Cannabinoids were present in small traces, with THC detected in only five varieties. The nutritional composition of hempseeds makes them a valuable ingredient for human and animal diets. The study highlights the importance of monitoring THC levels to ensure safety. The findings suggest that hempseeds are a rich source of nutrients and bioactive compounds, with potential applications in food and feed. The results emphasize the need for further research on antinutritional factors and bioactive compounds in hempseeds.A study analyzed the chemical composition of 29 industrial hempseed (Cannabis sativa L.) varieties, focusing on fatty acid profiles, amino acid content, mineral composition, and cannabinoid levels. The results showed significant variations among the varieties for all measured parameters. Proximate composition revealed that crude protein and fat contents ranged from 21.6–28.9% and 21.1–35.7%, respectively. Fatty acids included linoleic acid (52.79–57.13%), α-linolenic acid (12.62–20.24%), and oleic acid (11.08–17.81%). All essential amino acids were present, with arginine being abundant and lysine limiting. Mineral content varied, with potassium and iron as the main macro- and microminerals. Cannabinoids were present in small traces, with THC detected in only five varieties. The nutritional composition of hempseeds makes them a valuable ingredient for human and animal diets. The study highlights the importance of monitoring THC levels to ensure safety. The findings suggest that hempseeds are a rich source of nutrients and bioactive compounds, with potential applications in food and feed. The results emphasize the need for further research on antinutritional factors and bioactive compounds in hempseeds.