The article describes the development and application of coherent anti-Stokes Raman scattering (CARS) microscopy for in vivo imaging of tissue. CARS microscopy combines coherent anti-Stokes Raman scattering with video-rate microscopy to achieve high sensitivity and real-time imaging of tissue structures. The technique leverages the strong vibrational signals generated by CARS, which are particularly effective for imaging lipid-rich tissues. The authors demonstrate that backscattering of the forward-propagating CARS radiation in tissue generates a strong epi-CARS signal, enabling the visualization of subcellular structures in live mouse skin. This method provides unprecedented contrast and resolution, allowing for the detailed examination of sebaceous glands, corneocytes, and adipocytes. The study also highlights the potential of CARS microscopy for tracking chemical diffusion in skin and combining it with two-photon fluorescence microscopy to provide additional chemical information. The research underscores the potential of CARS microscopy for biomedical applications, particularly in tissue pathology and molecular imaging.The article describes the development and application of coherent anti-Stokes Raman scattering (CARS) microscopy for in vivo imaging of tissue. CARS microscopy combines coherent anti-Stokes Raman scattering with video-rate microscopy to achieve high sensitivity and real-time imaging of tissue structures. The technique leverages the strong vibrational signals generated by CARS, which are particularly effective for imaging lipid-rich tissues. The authors demonstrate that backscattering of the forward-propagating CARS radiation in tissue generates a strong epi-CARS signal, enabling the visualization of subcellular structures in live mouse skin. This method provides unprecedented contrast and resolution, allowing for the detailed examination of sebaceous glands, corneocytes, and adipocytes. The study also highlights the potential of CARS microscopy for tracking chemical diffusion in skin and combining it with two-photon fluorescence microscopy to provide additional chemical information. The research underscores the potential of CARS microscopy for biomedical applications, particularly in tissue pathology and molecular imaging.