Chitosan, a biopolymer derived from chitin, has emerged as a versatile and favorable material in tissue engineering and wound healing due to its biocompatibility, biodegradability, and antimicrobial properties. In tissue engineering, chitosan-based formulations mimic the extracellular matrix, promoting cell adhesion, proliferation, and differentiation. In wound healing, chitosan-based dressings maintain a moist environment, expedite wound closure, and prevent infections. These formulations also provide controlled release mechanisms for bioactive molecules and exhibit immunomodulatory properties to govern inflammatory reactions. Recent progress in chitosan-based formulations has significantly advanced tissue engineering and wound healing, offering promising outcomes for enhancing patient outcomes, reducing healing times, and minimizing complications. Future research is expected to lead to more sophisticated chitosan-based formulations for tissue repair and wound management, emphasizing its potential as a cornerstone in regenerative medicine and wound care.Chitosan, a biopolymer derived from chitin, has emerged as a versatile and favorable material in tissue engineering and wound healing due to its biocompatibility, biodegradability, and antimicrobial properties. In tissue engineering, chitosan-based formulations mimic the extracellular matrix, promoting cell adhesion, proliferation, and differentiation. In wound healing, chitosan-based dressings maintain a moist environment, expedite wound closure, and prevent infections. These formulations also provide controlled release mechanisms for bioactive molecules and exhibit immunomodulatory properties to govern inflammatory reactions. Recent progress in chitosan-based formulations has significantly advanced tissue engineering and wound healing, offering promising outcomes for enhancing patient outcomes, reducing healing times, and minimizing complications. Future research is expected to lead to more sophisticated chitosan-based formulations for tissue repair and wound management, emphasizing its potential as a cornerstone in regenerative medicine and wound care.