The study by Rami Moran and Dan Porath from Tel Aviv University, Israel, investigates the efficiency of extracting photosynthetic pigments from etiolated cucumber cotyledons using N,N-dimethylformamide (DMF) compared to acetone. The researchers found that DMF is more effective in extracting chlorophylls (Pchl and Chl a) from intact cotyledons, even when plant material is first ground and centrifuged. DMF extracts showed higher pigment yields and stability during storage. The specific absorption coefficient of Chl a in DMF was determined to be 83.89 g⁻¹ l⁻¹ at 664.5 nm. The results suggest that DMF simplifies and expedites the extraction process, avoiding pigment loss typically associated with grinding and centrifuging, which is particularly beneficial for low-pigment tissues.The study by Rami Moran and Dan Porath from Tel Aviv University, Israel, investigates the efficiency of extracting photosynthetic pigments from etiolated cucumber cotyledons using N,N-dimethylformamide (DMF) compared to acetone. The researchers found that DMF is more effective in extracting chlorophylls (Pchl and Chl a) from intact cotyledons, even when plant material is first ground and centrifuged. DMF extracts showed higher pigment yields and stability during storage. The specific absorption coefficient of Chl a in DMF was determined to be 83.89 g⁻¹ l⁻¹ at 664.5 nm. The results suggest that DMF simplifies and expedites the extraction process, avoiding pigment loss typically associated with grinding and centrifuging, which is particularly beneficial for low-pigment tissues.