The article "Choosing the Right Cell Line for Breast Cancer Research" by Deborah L Holliday and Valerie Speirs discusses the challenges and advancements in using breast cancer cell lines as models for research. Breast cancer is a complex disease with significant heterogeneity, which has been increasingly understood through gene expression profiling. The authors highlight the importance of cell lines in cancer research, noting their ability to provide an infinite supply of homogeneous cell populations. However, they also emphasize the limitations of using cell lines, particularly in reflecting the clinical heterogeneity of breast cancer. They review the development of various breast cancer cell lines, the molecular subtypes of breast cancer, and the need for more sophisticated models that incorporate the complex microenvironment of breast tumors. The article also discusses the use of three-dimensional cultures and xenograft models, emphasizing the importance of considering the appropriate cell culture conditions and the limitations of these models. Finally, the authors call for a more rational approach to selecting cell lines for research, based on the specific subgroups of breast cancer being studied, to improve translational research and patient outcomes.The article "Choosing the Right Cell Line for Breast Cancer Research" by Deborah L Holliday and Valerie Speirs discusses the challenges and advancements in using breast cancer cell lines as models for research. Breast cancer is a complex disease with significant heterogeneity, which has been increasingly understood through gene expression profiling. The authors highlight the importance of cell lines in cancer research, noting their ability to provide an infinite supply of homogeneous cell populations. However, they also emphasize the limitations of using cell lines, particularly in reflecting the clinical heterogeneity of breast cancer. They review the development of various breast cancer cell lines, the molecular subtypes of breast cancer, and the need for more sophisticated models that incorporate the complex microenvironment of breast tumors. The article also discusses the use of three-dimensional cultures and xenograft models, emphasizing the importance of considering the appropriate cell culture conditions and the limitations of these models. Finally, the authors call for a more rational approach to selecting cell lines for research, based on the specific subgroups of breast cancer being studied, to improve translational research and patient outcomes.