The Compilation of tRNA Sequences and Sequences of tRNA Genes is a searchable, periodically updated database of tRNA sequences, maintained at the Universität Bayreuth, Germany, and available at http://www.tRNA.uni-bayreuth.de and http://alpha.protres.ru/trnadbase. The database contains approximately 5800 tRNA gene sequences from 111 organisms, including archaea, bacteria, and eukaryotes. It includes a new Genomic tRNA Compilation with sequences from complete genomes published up to July 2003. The database also includes the Compilation of tRNA Sequences (561 entries) and the Compilation of tRNA Genes (up to 1998). The database allows users to search using multiple criteria and sequence templates, and provides statistical information on base occurrences in tRNA sequences, aiding in phylogenetic studies and identification of interaction elements with enzymes. The database is organized as MS Excel workbooks, with information split into indexed tables and summarized in main worksheets. Users can search for sequences and view them in cloverleaf format, with color-coded structural features. The database also includes sequence alignments compatible with tRNA phylogeny and structure. Modified nucleotides are denoted using ASCII signs other than A, C, G, T, and U. The database is accessible for research purposes and can be cited in projects using the compilation. It is supported by the Fonds der Chemischen Industrie and Universität Bayreuth. The database includes references to publications and is updated up to December 2002. The Compilation of tRNA Sequences and Genes are available in classical tabular form and in MS Excel format. The database supports statistical analysis of base occurrences and base-pairing preferences. It includes information on tRNA gene positions in genomes and organism taxonomy. The database is a valuable resource for researchers studying tRNA sequences, structure, and function.The Compilation of tRNA Sequences and Sequences of tRNA Genes is a searchable, periodically updated database of tRNA sequences, maintained at the Universität Bayreuth, Germany, and available at http://www.tRNA.uni-bayreuth.de and http://alpha.protres.ru/trnadbase. The database contains approximately 5800 tRNA gene sequences from 111 organisms, including archaea, bacteria, and eukaryotes. It includes a new Genomic tRNA Compilation with sequences from complete genomes published up to July 2003. The database also includes the Compilation of tRNA Sequences (561 entries) and the Compilation of tRNA Genes (up to 1998). The database allows users to search using multiple criteria and sequence templates, and provides statistical information on base occurrences in tRNA sequences, aiding in phylogenetic studies and identification of interaction elements with enzymes. The database is organized as MS Excel workbooks, with information split into indexed tables and summarized in main worksheets. Users can search for sequences and view them in cloverleaf format, with color-coded structural features. The database also includes sequence alignments compatible with tRNA phylogeny and structure. Modified nucleotides are denoted using ASCII signs other than A, C, G, T, and U. The database is accessible for research purposes and can be cited in projects using the compilation. It is supported by the Fonds der Chemischen Industrie and Universität Bayreuth. The database includes references to publications and is updated up to December 2002. The Compilation of tRNA Sequences and Genes are available in classical tabular form and in MS Excel format. The database supports statistical analysis of base occurrences and base-pairing preferences. It includes information on tRNA gene positions in genomes and organism taxonomy. The database is a valuable resource for researchers studying tRNA sequences, structure, and function.