The study presents the complete nucleotide sequence and comparative analysis of the linear chromosome of *Streptomyces avermitilis*, a microorganism known for producing antiparasitic agents like avermectins. The genome consists of 9,025,608 bases with an average GC content of 70.7%, encoding at least 7,574 potential open reading frames (ORFs). Thirty-five percent of these ORFs form 721 paralogous families, and 30 gene clusters related to secondary metabolite biosynthesis were identified. Comparative analysis with *Streptomyces coelicolor* A3(2) revealed a highly conserved 6.5-Mb internal region with essential genes but asymmetric structure at the *oriC* center. The terminal regions, which contain nonessential genes, showed less conservation. The study also highlights the presence of unique features such as two rpoA genes encoding RNA polymerase alpha subunits and numerous sigma factors. The linear genome structure and the distribution of genes related to secondary metabolism and defense mechanisms are discussed, providing insights into the genetic features of *Streptomyces* species.The study presents the complete nucleotide sequence and comparative analysis of the linear chromosome of *Streptomyces avermitilis*, a microorganism known for producing antiparasitic agents like avermectins. The genome consists of 9,025,608 bases with an average GC content of 70.7%, encoding at least 7,574 potential open reading frames (ORFs). Thirty-five percent of these ORFs form 721 paralogous families, and 30 gene clusters related to secondary metabolite biosynthesis were identified. Comparative analysis with *Streptomyces coelicolor* A3(2) revealed a highly conserved 6.5-Mb internal region with essential genes but asymmetric structure at the *oriC* center. The terminal regions, which contain nonessential genes, showed less conservation. The study also highlights the presence of unique features such as two rpoA genes encoding RNA polymerase alpha subunits and numerous sigma factors. The linear genome structure and the distribution of genes related to secondary metabolism and defense mechanisms are discussed, providing insights into the genetic features of *Streptomyces* species.