Multiple displacement amplification (MDA) is a method for amplifying the entire human genome with high uniformity and minimal bias. Unlike traditional whole genome amplification (WGA) methods such as degenerate oligonucleotide-primed PCR (DOP-PCR), MDA provides a more even representation of genomic regions, with amplification bias between eight chromosomal loci less than 3-fold, compared to 4–6 orders of magnitude for PCR-based WGA. MDA produces DNA products averaging over 10 kb in length and can generate 20–30 μg of product from as few as 1–10 copies of human genomic DNA. It can be performed directly from biological samples such as whole blood and tissue culture cells, making it suitable for various genetic analyses including genotyping of single nucleotide polymorphisms (SNPs), chromosome painting, Southern blotting, restriction fragment length polymorphism (RFLP) analysis, subcloning, and DNA sequencing. MDA is an isothermal, strand-displacing amplification process that uses phi29 DNA polymerase and exonuclease-resistant primers. It is highly efficient and reliable, offering significant implications for genetic studies, forensics, diagnostics, and long-term sample storage. MDA-based WGA is simple and robust, capable of accurate amplification from as few as 10 cells. It uniformly amplifies the human genome directly from whole blood without the need for DNA purification. MDA provides a more accurate representation of genomic sequences compared to PCR-based methods, with locus representation ranging from 80–225% of the starting genomic DNA. This method is particularly useful for applications requiring complete genome coverage with minimal amplification bias, such as genome-wide SNP genotyping, comparative genome hybridization, and cytogenetic testing. MDA is also valuable for amplifying DNA from limited patient samples such as needle biopsy material or amniocentesis samples. The method has been shown to be effective for DNA probe preparation for comparative genome hybridization, karyotyping, and chip-based genetic analysis. MDA is a promising alternative to PCR-based WGA methods, offering a more uniform and accurate amplification of genomic DNA.Multiple displacement amplification (MDA) is a method for amplifying the entire human genome with high uniformity and minimal bias. Unlike traditional whole genome amplification (WGA) methods such as degenerate oligonucleotide-primed PCR (DOP-PCR), MDA provides a more even representation of genomic regions, with amplification bias between eight chromosomal loci less than 3-fold, compared to 4–6 orders of magnitude for PCR-based WGA. MDA produces DNA products averaging over 10 kb in length and can generate 20–30 μg of product from as few as 1–10 copies of human genomic DNA. It can be performed directly from biological samples such as whole blood and tissue culture cells, making it suitable for various genetic analyses including genotyping of single nucleotide polymorphisms (SNPs), chromosome painting, Southern blotting, restriction fragment length polymorphism (RFLP) analysis, subcloning, and DNA sequencing. MDA is an isothermal, strand-displacing amplification process that uses phi29 DNA polymerase and exonuclease-resistant primers. It is highly efficient and reliable, offering significant implications for genetic studies, forensics, diagnostics, and long-term sample storage. MDA-based WGA is simple and robust, capable of accurate amplification from as few as 10 cells. It uniformly amplifies the human genome directly from whole blood without the need for DNA purification. MDA provides a more accurate representation of genomic sequences compared to PCR-based methods, with locus representation ranging from 80–225% of the starting genomic DNA. This method is particularly useful for applications requiring complete genome coverage with minimal amplification bias, such as genome-wide SNP genotyping, comparative genome hybridization, and cytogenetic testing. MDA is also valuable for amplifying DNA from limited patient samples such as needle biopsy material or amniocentesis samples. The method has been shown to be effective for DNA probe preparation for comparative genome hybridization, karyotyping, and chip-based genetic analysis. MDA is a promising alternative to PCR-based WGA methods, offering a more uniform and accurate amplification of genomic DNA.