The article discusses the use of μManager, a free, open-source software for controlling automated microscope equipment. It provides detailed protocols for using μManager, including setting up hardware, configuring devices, performing multi-dimensional acquisitions, and using the image viewer. The software supports a wide range of hardware from various manufacturers and runs on Windows, Mac, and Linux systems. It includes a user-friendly graphical interface, advanced scripting capabilities, and a plugin system for extending functionality. The article also covers hardware connections, pixel size calibration, and integration with external programming languages like Python and MATLAB. μManager is designed to be modular, allowing for easy expansion and customization. It is particularly useful for both expert and non-expert users, enabling automated and reproducible microscopy experiments. The software is continuously being developed to improve usability, expand features, and support a broader range of devices. The article concludes that μManager has the potential to make advanced microscopy techniques more accessible to researchers through its open-source nature and modular design.The article discusses the use of μManager, a free, open-source software for controlling automated microscope equipment. It provides detailed protocols for using μManager, including setting up hardware, configuring devices, performing multi-dimensional acquisitions, and using the image viewer. The software supports a wide range of hardware from various manufacturers and runs on Windows, Mac, and Linux systems. It includes a user-friendly graphical interface, advanced scripting capabilities, and a plugin system for extending functionality. The article also covers hardware connections, pixel size calibration, and integration with external programming languages like Python and MATLAB. μManager is designed to be modular, allowing for easy expansion and customization. It is particularly useful for both expert and non-expert users, enabling automated and reproducible microscopy experiments. The software is continuously being developed to improve usability, expand features, and support a broader range of devices. The article concludes that μManager has the potential to make advanced microscopy techniques more accessible to researchers through its open-source nature and modular design.