Conductive polymer-based hydrogels (CP HGs) are promising materials for wearable electrochemical biosensors due to their flexibility, biocompatibility, and excellent electrical conductivity. This review summarizes recent advancements in polymer-hydrogel-based wearable electrochemical biosensors over the past five years. Initially used as biomolecule carriers, these sensors have expanded to include non-enzymatic sensors, facilitated by the integration of nanomaterials like metals, metal oxides, and carbon-based materials. Challenges remain, such as limited strain-sensing range, hysteresis, dehydration-induced failure, and surface malfunction during manufacturing. CP HGs combine the benefits of conductive polymers and hydrogels, offering extensive surface area, electrical conductivity, and flexibility. They are being explored to address challenges in developing wearable biosensors, including finding suitable electrode materials and improving flexibility, stretchability, self-healing, and transparency. The review also discusses the fabrication of nanocomposite conductive polymer hydrogels and their varied conductivity mechanisms in electrochemical sensing applications. CP HGs are being developed for various applications, including strain sensors, biosensors, and electronic skin. The review highlights the importance of electrical conductivity, mechanical properties, self-healing, adhesion, and biocompatibility in the development of CP HGs for wearable biosensors. The review also discusses the applications of wearable electrochemical biosensors (WEBSs) in glucose monitoring, with a focus on non-invasive detection of glucose in sweat, tears, and saliva. The review emphasizes the need for sensitive sensing technologies to detect glucose in sweat despite low concentrations and contamination. Recent advancements in WEBSs include the development of hydrogel-based sensors with high sensitivity, selectivity, and long-term stability. The review concludes that CP HGs are a promising candidate for wearable biosensors due to their unique properties and potential applications in healthcare and biomedical technologies.Conductive polymer-based hydrogels (CP HGs) are promising materials for wearable electrochemical biosensors due to their flexibility, biocompatibility, and excellent electrical conductivity. This review summarizes recent advancements in polymer-hydrogel-based wearable electrochemical biosensors over the past five years. Initially used as biomolecule carriers, these sensors have expanded to include non-enzymatic sensors, facilitated by the integration of nanomaterials like metals, metal oxides, and carbon-based materials. Challenges remain, such as limited strain-sensing range, hysteresis, dehydration-induced failure, and surface malfunction during manufacturing. CP HGs combine the benefits of conductive polymers and hydrogels, offering extensive surface area, electrical conductivity, and flexibility. They are being explored to address challenges in developing wearable biosensors, including finding suitable electrode materials and improving flexibility, stretchability, self-healing, and transparency. The review also discusses the fabrication of nanocomposite conductive polymer hydrogels and their varied conductivity mechanisms in electrochemical sensing applications. CP HGs are being developed for various applications, including strain sensors, biosensors, and electronic skin. The review highlights the importance of electrical conductivity, mechanical properties, self-healing, adhesion, and biocompatibility in the development of CP HGs for wearable biosensors. The review also discusses the applications of wearable electrochemical biosensors (WEBSs) in glucose monitoring, with a focus on non-invasive detection of glucose in sweat, tears, and saliva. The review emphasizes the need for sensitive sensing technologies to detect glucose in sweat despite low concentrations and contamination. Recent advancements in WEBSs include the development of hydrogel-based sensors with high sensitivity, selectivity, and long-term stability. The review concludes that CP HGs are a promising candidate for wearable biosensors due to their unique properties and potential applications in healthcare and biomedical technologies.