The study investigates the kinetics, control, and efficiency of nisin-induced expression in *Lactococcus lactis* using the nisA promoter region. The research focuses on transcriptional and translational fusions to the gasA reporter gene. In the nisin-producing strain NZ9700, β-glucuronidase activity increases rapidly after mid-exponential growth until it reaches a maximum at the start of the stationary phase. In strains NZ9800 (nisA deletion) and NZ3900 (nisRK genes integrated), β-glucuronidase activity is linearly dependent on nisin concentration. The translational fusion vector, which fuses the gasA gene to the nisA promoter, yields up to six times more β-glucuronidase activity than the transcriptional fusion vector. The β-glucuronidase activity is 25 times higher in strain NZ3900 compared to strain NZ9800. The translational fusion vector was used to produce aminopeptidase N, achieving up to 47% of the total intracellular protein. These results demonstrate the potential of the nisin-inducible expression system for overproduction of desired proteins in *L. lactis*.The study investigates the kinetics, control, and efficiency of nisin-induced expression in *Lactococcus lactis* using the nisA promoter region. The research focuses on transcriptional and translational fusions to the gasA reporter gene. In the nisin-producing strain NZ9700, β-glucuronidase activity increases rapidly after mid-exponential growth until it reaches a maximum at the start of the stationary phase. In strains NZ9800 (nisA deletion) and NZ3900 (nisRK genes integrated), β-glucuronidase activity is linearly dependent on nisin concentration. The translational fusion vector, which fuses the gasA gene to the nisA promoter, yields up to six times more β-glucuronidase activity than the transcriptional fusion vector. The β-glucuronidase activity is 25 times higher in strain NZ3900 compared to strain NZ9800. The translational fusion vector was used to produce aminopeptidase N, achieving up to 47% of the total intracellular protein. These results demonstrate the potential of the nisin-inducible expression system for overproduction of desired proteins in *L. lactis*.