Copper is an essential metal for plant growth and development, but it can also be toxic. Copper participates in various physiological processes and is a cofactor for many metalloproteins. However, excess copper can inhibit plant growth and impair important cellular processes, such as photosynthetic electron transport. Plants have evolved strategies to regulate copper homeostasis, preventing its accumulation in the reactive form and ensuring proper delivery to target metalloproteins. Recent studies have identified genes encoding potential copper transporters, and this review provides an overview of current understanding of copper toxicity and tolerance in plants, including recent findings on copper trafficking, detoxification factors, transporters, and chaperones. Key mechanisms involve the use of P-type ATPases, COPT copper transporters, and copper chaperones to manage copper uptake, distribution, and detoxification within the plant.Copper is an essential metal for plant growth and development, but it can also be toxic. Copper participates in various physiological processes and is a cofactor for many metalloproteins. However, excess copper can inhibit plant growth and impair important cellular processes, such as photosynthetic electron transport. Plants have evolved strategies to regulate copper homeostasis, preventing its accumulation in the reactive form and ensuring proper delivery to target metalloproteins. Recent studies have identified genes encoding potential copper transporters, and this review provides an overview of current understanding of copper toxicity and tolerance in plants, including recent findings on copper trafficking, detoxification factors, transporters, and chaperones. Key mechanisms involve the use of P-type ATPases, COPT copper transporters, and copper chaperones to manage copper uptake, distribution, and detoxification within the plant.