Cumin (Cuminum cyminum L.) seeds have shown potential in accelerating wound healing in rats, with possible molecular mechanisms involving antioxidant and anti-inflammatory effects. The study evaluated the wound healing potential of methanolic extract of cumin seeds (CCS) in Sprague Dawley rats. Rats were divided into groups and treated with normal saline, intrasite gel, or CCS at 250 and 500 mg/kg. Wound area and closure percentage were measured, and tissues were analyzed histologically, immunohistochemically, and biochemically. CCS treatment significantly reduced wound size and increased wound contraction compared to vehicle rats. Histopathological analysis showed increased collagen fiber, fibroblast density, and reduced inflammatory cells in CCS-treated rats. TGF-β1 expression was significantly higher in CCS-treated rats, indicating enhanced angiogenesis and fibroblast transformation. CCS also showed antioxidant effects, with increased SOD and CAT enzyme levels and reduced MDA levels, suggesting reduced oxidative stress. Hydroxyproline levels were significantly higher in CCS-treated rats, indicating increased collagen synthesis and faster wound healing. The study concluded that CCS has potential as a natural source for wound healing due to its antioxidant, anti-inflammatory, and collagen-stimulating properties. The research highlights the therapeutic potential of cumin seeds in wound healing, with implications for the development of natural remedies.Cumin (Cuminum cyminum L.) seeds have shown potential in accelerating wound healing in rats, with possible molecular mechanisms involving antioxidant and anti-inflammatory effects. The study evaluated the wound healing potential of methanolic extract of cumin seeds (CCS) in Sprague Dawley rats. Rats were divided into groups and treated with normal saline, intrasite gel, or CCS at 250 and 500 mg/kg. Wound area and closure percentage were measured, and tissues were analyzed histologically, immunohistochemically, and biochemically. CCS treatment significantly reduced wound size and increased wound contraction compared to vehicle rats. Histopathological analysis showed increased collagen fiber, fibroblast density, and reduced inflammatory cells in CCS-treated rats. TGF-β1 expression was significantly higher in CCS-treated rats, indicating enhanced angiogenesis and fibroblast transformation. CCS also showed antioxidant effects, with increased SOD and CAT enzyme levels and reduced MDA levels, suggesting reduced oxidative stress. Hydroxyproline levels were significantly higher in CCS-treated rats, indicating increased collagen synthesis and faster wound healing. The study concluded that CCS has potential as a natural source for wound healing due to its antioxidant, anti-inflammatory, and collagen-stimulating properties. The research highlights the therapeutic potential of cumin seeds in wound healing, with implications for the development of natural remedies.