This study investigates the wound healing potential of Cuminum cyminum L. (cumin) seeds in rats. The methanolic extract of cumin seeds was evaluated for its effects on wound healing in a group of 24 Sprague Dawley rats. The rats were divided into four groups: Group A received normal saline, Group B received intrasite gel, Group C received 0.2 mL of 250 mg/kg cumin extract, and Group D received 0.2 mL of 500 mg/kg cumin extract. After 14 days, the wound area and closure percentage were measured, and histopathological, immunohistochemical, and biochemical analyses were conducted. The acute toxicity trial showed no significant physiological changes or mortality in the rats treated with cumin extract. Cumin treatment significantly reduced wound size and increased wound contraction compared to the vehicle group. Histopathological analysis revealed increased collagen fiber formation, fibroblast proliferation, and reduced inflammatory cell infiltration in the cumin-treated wounds. Immunohistochemical analysis showed increased TGF-β1 expression, indicating enhanced tissue remodeling and regeneration. Biochemical analyses demonstrated increased antioxidant enzyme activity (SOD and CAT) and reduced lipid peroxidation (MDA) levels, suggesting anti-oxidant properties. Additionally, cumin treatment significantly increased hydroxyproline content, indicating higher collagen synthesis and faster wound healing. The study concludes that cumin extract has significant wound healing potential, with 500 mg/kg being the most effective dose. The mechanisms involved include increased collagen formation, fibroblast proliferation, reduced inflammation, and enhanced antioxidant activity. These findings suggest that cumin could be a promising natural therapeutic agent for wound healing.This study investigates the wound healing potential of Cuminum cyminum L. (cumin) seeds in rats. The methanolic extract of cumin seeds was evaluated for its effects on wound healing in a group of 24 Sprague Dawley rats. The rats were divided into four groups: Group A received normal saline, Group B received intrasite gel, Group C received 0.2 mL of 250 mg/kg cumin extract, and Group D received 0.2 mL of 500 mg/kg cumin extract. After 14 days, the wound area and closure percentage were measured, and histopathological, immunohistochemical, and biochemical analyses were conducted. The acute toxicity trial showed no significant physiological changes or mortality in the rats treated with cumin extract. Cumin treatment significantly reduced wound size and increased wound contraction compared to the vehicle group. Histopathological analysis revealed increased collagen fiber formation, fibroblast proliferation, and reduced inflammatory cell infiltration in the cumin-treated wounds. Immunohistochemical analysis showed increased TGF-β1 expression, indicating enhanced tissue remodeling and regeneration. Biochemical analyses demonstrated increased antioxidant enzyme activity (SOD and CAT) and reduced lipid peroxidation (MDA) levels, suggesting anti-oxidant properties. Additionally, cumin treatment significantly increased hydroxyproline content, indicating higher collagen synthesis and faster wound healing. The study concludes that cumin extract has significant wound healing potential, with 500 mg/kg being the most effective dose. The mechanisms involved include increased collagen formation, fibroblast proliferation, reduced inflammation, and enhanced antioxidant activity. These findings suggest that cumin could be a promising natural therapeutic agent for wound healing.