Cyclodextrins (CDs) are cyclic oligosaccharides composed of glucose units linked by α-(1,4) glucosidic bonds. The three natural CDs—α-, β-, and γ-CDs—differ in size and solubility, with α-CD containing 6 glucose units, β-CD 7, and γ-CD 8. CDs have a toroidal structure with a hydrophilic exterior and a hydrophobic inner cavity, allowing them to encapsulate hydrophobic molecules, forming inclusion complexes. Their ability to modify the physical, chemical, and biological properties of guest molecules makes them valuable in various industries, including pharmaceuticals, food, cosmetics, and biotechnology. CDs are biocompatible, biodegradable, and cost-effective, leading to their widespread use in drug delivery, solubilization, and controlled release systems. Despite their importance, research into their structure and properties continues, with a focus on enhancing their complexing capacity and exploring new applications. Recent studies highlight their potential in environmental remediation, nanotechnology, and medical applications. The Special Issue "Cyclodextrins: Properties and Applications" presents 14 research papers covering topics such as the use of CDs in drug formulations, their interaction with various compounds, and their role in nanotechnology and biomedicine. Contributions include the development of eye drop formulations with voriconazole, the formation of drug delivery systems for melphalan, the encapsulation of α-tocopherol, and the use of CDs in the treatment of acute myeloid leukemia. The study also explores the conformational properties of CDs, their role in supramolecular complexes, and their application in advanced drug delivery systems. Overall, CDs continue to be a subject of significant research due to their versatility and wide range of applications.Cyclodextrins (CDs) are cyclic oligosaccharides composed of glucose units linked by α-(1,4) glucosidic bonds. The three natural CDs—α-, β-, and γ-CDs—differ in size and solubility, with α-CD containing 6 glucose units, β-CD 7, and γ-CD 8. CDs have a toroidal structure with a hydrophilic exterior and a hydrophobic inner cavity, allowing them to encapsulate hydrophobic molecules, forming inclusion complexes. Their ability to modify the physical, chemical, and biological properties of guest molecules makes them valuable in various industries, including pharmaceuticals, food, cosmetics, and biotechnology. CDs are biocompatible, biodegradable, and cost-effective, leading to their widespread use in drug delivery, solubilization, and controlled release systems. Despite their importance, research into their structure and properties continues, with a focus on enhancing their complexing capacity and exploring new applications. Recent studies highlight their potential in environmental remediation, nanotechnology, and medical applications. The Special Issue "Cyclodextrins: Properties and Applications" presents 14 research papers covering topics such as the use of CDs in drug formulations, their interaction with various compounds, and their role in nanotechnology and biomedicine. Contributions include the development of eye drop formulations with voriconazole, the formation of drug delivery systems for melphalan, the encapsulation of α-tocopherol, and the use of CDs in the treatment of acute myeloid leukemia. The study also explores the conformational properties of CDs, their role in supramolecular complexes, and their application in advanced drug delivery systems. Overall, CDs continue to be a subject of significant research due to their versatility and wide range of applications.