Cyclodextrins (CDs) are cyclic oligosaccharides composed of glucose units linked by α-(1,4) glucosidic bonds. The three natural CDs, α-, β-, and γ-CDs, have 6, 7, and 8 glucose units, respectively. CDs have a toroidal structure with a hydrophilic exterior and a hydrophobic inner cavity, allowing them to encapsulate hydrophobic molecules, forming inclusion complexes. Their unique properties make them valuable in various industries, including pharmaceuticals, food, cosmetics, and textiles. CDs are biocompatible, biodegradable, and cost-effective, enabling their use in drug delivery, improving solubility and bioavailability, and as catalysts in chemical reactions. CDs have been studied extensively for their ability to enhance the solubility and stability of drugs, improve the properties of food products, and serve as carriers for various substances. Recent research highlights their potential in controlled release systems, nanotechnology, and environmental applications. Despite their widespread use, further research is needed to fully understand their structure and properties, as well as to develop new applications. This Special Issue presents 14 research papers and reviews covering the structure, properties, and applications of CDs. Topics include the development of eye drop formulations, interactions between CDs and various compounds, the formation of solid complexes for drug delivery, and the use of CDs in nanotechnology and environmental applications. The studies demonstrate the versatility of CDs in enhancing drug solubility, improving bioavailability, and enabling new applications in medicine, food, and environmental science. The research also addresses the potential toxicity of CDs and methods to mitigate it, ensuring their safe use in various industries. Overall, CDs continue to be a promising area of research with significant potential for future applications.Cyclodextrins (CDs) are cyclic oligosaccharides composed of glucose units linked by α-(1,4) glucosidic bonds. The three natural CDs, α-, β-, and γ-CDs, have 6, 7, and 8 glucose units, respectively. CDs have a toroidal structure with a hydrophilic exterior and a hydrophobic inner cavity, allowing them to encapsulate hydrophobic molecules, forming inclusion complexes. Their unique properties make them valuable in various industries, including pharmaceuticals, food, cosmetics, and textiles. CDs are biocompatible, biodegradable, and cost-effective, enabling their use in drug delivery, improving solubility and bioavailability, and as catalysts in chemical reactions. CDs have been studied extensively for their ability to enhance the solubility and stability of drugs, improve the properties of food products, and serve as carriers for various substances. Recent research highlights their potential in controlled release systems, nanotechnology, and environmental applications. Despite their widespread use, further research is needed to fully understand their structure and properties, as well as to develop new applications. This Special Issue presents 14 research papers and reviews covering the structure, properties, and applications of CDs. Topics include the development of eye drop formulations, interactions between CDs and various compounds, the formation of solid complexes for drug delivery, and the use of CDs in nanotechnology and environmental applications. The studies demonstrate the versatility of CDs in enhancing drug solubility, improving bioavailability, and enabling new applications in medicine, food, and environmental science. The research also addresses the potential toxicity of CDs and methods to mitigate it, ensuring their safe use in various industries. Overall, CDs continue to be a promising area of research with significant potential for future applications.