DIANA-TarBase v8 is a comprehensive database that catalogs experimentally supported microRNA (miRNA) targets, featuring over 1 million entries and approximately 670,000 unique miRNA-target pairs. The database integrates interactions supported by 33 experimental methodologies applied to 600 cell types/tissues under 451 experimental conditions. It includes information on cell-type-specific miRNA-gene regulation and hundreds of thousands of miRNA-binding locations. A new module allows users to browse interactions through various filtering combinations, facilitating the retrieval of positive and negative miRNA targets per species, methodology, cell type, and tissue. The database also incorporates a ranking system based on the robustness of supporting methodologies and provides statistics, pie charts, and interactive bar plots to visualize the content. The interface is user-friendly, offering flexible options for different queries. DIANA-TarBase v8 represents a significant advancement in miRNA research, providing a valuable resource for both experimental and computational studies.DIANA-TarBase v8 is a comprehensive database that catalogs experimentally supported microRNA (miRNA) targets, featuring over 1 million entries and approximately 670,000 unique miRNA-target pairs. The database integrates interactions supported by 33 experimental methodologies applied to 600 cell types/tissues under 451 experimental conditions. It includes information on cell-type-specific miRNA-gene regulation and hundreds of thousands of miRNA-binding locations. A new module allows users to browse interactions through various filtering combinations, facilitating the retrieval of positive and negative miRNA targets per species, methodology, cell type, and tissue. The database also incorporates a ranking system based on the robustness of supporting methodologies and provides statistics, pie charts, and interactive bar plots to visualize the content. The interface is user-friendly, offering flexible options for different queries. DIANA-TarBase v8 represents a significant advancement in miRNA research, providing a valuable resource for both experimental and computational studies.