DIANA-miRPath v3.0 is an advanced online software suite designed to assess the regulatory roles of microRNAs (miRNAs) and identify controlled pathways. The new version of the web server supports functional annotation of one or more miRNAs using standard, unbiased empirical, and meta-analysis statistics. It incorporates over 600,000 experimentally supported miRNA targets from DIANA-TarBase v7.0, enabling users to combine in silico predicted targets with high-quality experimental interactions. Key features include a redesigned Reverse Search module for identifying miRNAs controlling specific pathways or belonging to specific Gene Ontology (GO) categories, support for KEGG molecular pathways, and multiple GO annotations in seven species. The user interface has been enhanced to offer more options and advanced visualizations, making it a comprehensive tool for miRNA research.DIANA-miRPath v3.0 is an advanced online software suite designed to assess the regulatory roles of microRNAs (miRNAs) and identify controlled pathways. The new version of the web server supports functional annotation of one or more miRNAs using standard, unbiased empirical, and meta-analysis statistics. It incorporates over 600,000 experimentally supported miRNA targets from DIANA-TarBase v7.0, enabling users to combine in silico predicted targets with high-quality experimental interactions. Key features include a redesigned Reverse Search module for identifying miRNAs controlling specific pathways or belonging to specific Gene Ontology (GO) categories, support for KEGG molecular pathways, and multiple GO annotations in seven species. The user interface has been enhanced to offer more options and advanced visualizations, making it a comprehensive tool for miRNA research.